6-Chloropurine CAS: 87-42-3 Phofo e mosehla e khanyang
| Nomoro ea lethathamo | XD90547 |
| Lebitso la Sehlahiswa | 6-Chloropurine |
| CAS | 87-42-3 |
| Foromo ea limolek'hule | C5H3ClN4 |
| Boima ba Molek'hule | 154.557 |
| Lintlha tsa polokelo | 2 ho isa ho 8 °C |
| Khoutu ea Lekhetho e Kopantsoeng | 2933990090 |
Tlhaloso ea Sehlahisoa
| Ponahalo | Phofo e mosehla e khanyang |
| Tlhahlobo | 99% |
Haufinyane tjena re tlalehile hore pyrene nucleotide e kentsoe ka mokhoa o ikhethileng khahlano le sebaka sa abasic, 3'-T ea thymine dimer, le metheo e sa senyehang haholo ke tomoso DNA polymerase eta (pol eta).Hobane pyrene ke molek'hule e se nang polar e se nang bokhoni ba H-bonding, katleho e phahameng ka tsela e sa tloaelehang ea ho kenngoa ha dPMP e hlahisoa ke bokhoni ba eona bo phahameng ba ho bokellana ha motheo, 'me e totobatsa bohlokoa ba motheo oa stacking ha ho khethoa li-nucleotide ke pol eta.Ho batlisisa karolo ea H-bonding le base pair geometry khethong ea li-nucleotide ke pol eta, re ile ra etsa qeto ea ho kenya letsoho ha li-nucleotide tse fetotsoeng 2,6-diaminopurine, 2-aminopurine, 6-chloropurine, le inosine etsa palo e fapaneng ea li-bond tsa H ka motheo oa template ho latela geometry ea pair.Watson-Crick base pairing e bonahala e phetha karolo ea bohlokoa ho khethoeng ha li-nucleotide analogues bakeng sa ho kenngoa khahlanong le C le T joalokaha ho pakoa ke ho fokotseha ha matla a ho kenya letsoho ka ho fokotseha ha palo ea Watson-Crick H-bond le keketseho ea palo ea litšebelisano tsa bafani le ba amohelang le ba amohelang.Khetho ea ho kenya nucleotide e kholo ho feta 5'-T ho feta 3'-T ea thymine dimer, ho latela mosebetsi o fetileng o bontšang hore 5'-T e tšoaroa ka thata ho feta 3'-T.Ho feta moo, ho kenyeletsoa ha A khahlano le Ts ea dimer ka bobeli ho bonahala ho kenelletse lipakeng ke Watson-Crick base pairing eseng ka Hoogsteen base pairing ho ipapisitsoe le mats'oafo a batlang a ts'oana a A le 7-deaza-A, a ho qetela a haelloang ke H- bokhoni ba ho kopanya ho N7.Matla a lekanyelitsoeng a ho kenya li-nucleotide tse ka etsang lipara tsa motheo tsa Watson-Crick li tšoana le tsa sekhechana sa Klenow, athe sekhechana sa Klenow sona se khetholla ka matla ho feta ho se lumellane, ho latela khetho ea sona e kholoanyane.Liphetho tsena li totobatsa bohlokoa ba H-bonding le Watson-Crick base pair geometry khethong ea li-nucleotide ka bobeli ba pol eta le Klen Ow fragment, le karolo e nyane ea khetho ea sebopeho ho kenngoeng ke pol eta ka lebaka la ho buleha le ho fokola ha eona. tšitiso ea sebaka se sebetsang.
