Lihlahisoa

Ho lekanya ka ho hlaka metabolite tse 'maloa tsa 5-fluorouracil (5-FU) le li-nucleotide tse peli tsa monophosphate, re thehile mokhoa oa mantlha o thehiloeng ho mokelikeli oa chromatography-tandem mass spectrometry (LC-MS/MS).Teko ena e lumelletse boikemisetso ba: (i) tlhahiso ea intracellular ea 5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP) ho tloha 5-FU kapa 5-fluoro-2'-deoxyuridine (5-FdUrd) , (ii) tšusumetso ea 5-FdUMP concentration ho intracellular 2′-deoxyuridine-5′-monophosphate (dUMP)/thymidine-5′-monophosphate (TMP) ratio, le (iii) tekanyo ea secretion ea 5-FdUMP le 5-FU ho tsoa liseleng tse entsoeng ke batho ke lipalangoang tsa ABC.Tlas'a maemo a rona a liteko, lisele li ne li kenngoa ka 5-FU kapa 5-FUrd.Ka mor'a moo, liprotheine tsa cellular li ile tsa hlahisoa ke methanol.Mokhoa ona o ile oa fana ka pholiso e phahameng ea ho ntšoa.Ntle le moo, ho lekanya secretion ea 5-FU le 5-FdUMP ho tsoa liseleng, re ile ra etsa quantification ea limolek'hule tsena ka mokhoa oa setso.Mecha ea litaba e ne e kentsoe ka kotloloho (5-FU) kapa e ile ea ekoa ka mokhoa o tiileng ho sebelisoa khatriji ea Oasis Wax (5-FdUMP).Karohano ea li-analyte e entsoe ka kholomo ea dC18 Atlantis 3.5 μm, (100 mm × 2.1 mm id) e nang le mokhoa oa isocratic o sebelisang ammonium formate buffer/methanol/water (5/5/90, v/v) joalo ka mobile phase.Nako ea ho matha ha ea ka ea feta 6.2 min.Li-analyte li ne li ionized ka sebopeho sa electrospray tlas'a mokhoa o mobe oa ion.Re netefalitse mokhoa ona ho feta 2.5-150 ng mL -1 ho latela metsoako.Phapang ea ka hare le lipakeng tsa liteko e ne e le tlase ho feta 10% matsatsing a supileng.Lik'hemik'hale tsohle li ne li tsitsitse ka liseleng kapa moetlong oa setso ha lisampole li ne li bolokoa ho −20 °C bonyane libeke tse peli, le ka mor'a lipotoloho tse tharo tsa ho qhibiliha.Ha ho phello ea matrix e ileng ea bonoa mecheng ea litaba ka bobeli.