Lihlahisoa

Infrared spectroscopy e sebelisitsoe ho etsa 'mapa oa litšebelisano tsa protheine ea substrate: liphetoho tse amanang le sarcoplasmic reticulum Ca (2+) -ATPase holim'a nucleotide binding le ATPase phosphorylation li ile tsa hlahlojoa ho sebelisoa substrate ATP le ATP analogues (2'-deoxy-ATP, 3). '-deoxy-ATP, le inosine 5'-triphosphate), tse ileng tsa fetoloa lihlopheng tse itseng tse sebetsang tsa substrate.Liphetoho ho 2'-OH, 3'-OH, le sehlopha sa amino sa adenine se fokotsa tekanyo ea phetoho ea conformational e tlamang ea ATPase, ka liphello tse matla haholo tse hlokometsoeng bakeng sa tse peli tse qetellang.Sena se bonts'a kutloisiso ea sebopeho sa nucleotide-ATPase e rarahaneng ho sebelisana ka bomong lipakeng tsa nucleotide le ATPase.Lihlopha tsohle tse ithutoang li bohlokoa bakeng sa ho tlama le ho sebelisana ha sehlopha sa ligand se fanoeng le ATPase ho itšetlehile ka litšebelisano tsa lihlopha tse ling tsa ligand.Phosphorylation ea ATPase e ile ea hlokomeloa bakeng sa ITP le 2'-deoxy-ATP, empa eseng bakeng sa 3'-deoxy-ATP.Ha ho na kamano e tobileng pakeng tsa tekanyo ea phetoho ea conformational holim'a ho tlama ha nucleotide le tekanyo ea phosphorylation e bontšang hore tekanyo e feletseng ea phetoho e entsoeng ke ATP ha e tlamehe bakeng sa phosphorylation.Joalokaha ho hlokometsoe ka nucleotide-ATPase complex, conformation ea pele ea phosphorylated ATPase intermediate E1PCa (2) e boetse e itšetlehile ka nucleotide, e bontšang hore linaha tsa ATPase li na le conformation e fokolang ho feta kamoo ho neng ho lebeletsoe kateng pele.