Mofuta oa beta-glucosidase (bgl3) ho tsoa ho Streptomyces sp.QM-B814 (American Type Culture Collection 11238) e entsoe ka tlatsetso e sebetsang ea beta-glucosidase-negative mutant ea Streptomyces lividans.Foreimi e balang e bulehileng ea li-nucleotide tse 1440 tse khoute polypeptide ea 479 amino acid e fumanoe ka tatellano.Protheine e kentsoeng (Bgl3) e bonts'a ho tšoana ho hoholo (boitsebahatso bo fetang 45%) le beta-glycosidase ho tsoa ho lelapa-1 glycosyl hydrolases.Enzyme e hlophisitsoeng, e hloekisitsoeng ka mor'a ho na ha ammonium sulphate le mehato e 'meli ea chromatographic, ke monomeric e nang le boima ba molek'hule 52.6 kDa, joalo ka ha ho laetsoe ke mass spectrometry, le ntlha ea isoelectric ea pI 4.4.Enzyme e bonahala e le beta-glucosidase e nang le sebopeho se pharaletseng sa substrate, e sebetsa ho cellooligomers, 'me e etsa liphetoho tsa transglycosylation.Maemo a hakanyetsoang a bonahalang a Km bakeng sa p-nitrophenyl-beta-D-glucopyranoside le cellobiose ke 0.27 mM le 7.9 mM, ka ho latellana.Boleng ba Ki bakeng sa tsoekere le delta-gluconolactone, ho sebelisoa p-nitrophenyl-beta-D-glucopyranoside e le substrate, ke 65 mM le 0.08 mM, ka ho latellana.Enzyme e hloekisitsoeng e na le pH optimum ea pH 6.5 mme mocheso o nepahetseng bakeng sa ts'ebetso ke likhato tse 50.
