ONPG CAS:369-07-3 98.0% Min White To Off -White Powder
| Nomoro ea lethathamo | XD90006 |
| CAS | 369-07-3 |
| Lebitso la Sehlahiswa | ONPG(2-Nitrophenyl-beta-D-galactopyranoside) |
| Foromo ea limolek'hule | C12H15NO8 |
| Boima ba Molek'hule | 301.25 |
| Lintlha tsa polokelo | 2 ho isa ho 8 °C |
| Khoutu ea Lekhetho e Kopantsoeng | 29400000 |
Tlhaloso ea Sehlahisoa
| Purity (HPLC) | Min.98.0% |
| Ponahalo | Bosoeu ho isa ho phofshoana e tšoeu |
| Tharollo(1% ka Metsing) | Motsoako o hlakileng, o se nang 'mala ho isa ho o mosehla hanyane |
| Litaba tsa Metsi(Karl Fisher) | Max.0.5% |
| Potoloho e khethehileng ea mahlo [α]D20(c=1, H2O) | - 65.0 ° C ho -73.0 ° C |
Puisano ka teko ea ONPG (teko ea β-galactosidase)
Lipotso li 'nile tsa phahamisoa khafetsa haufinyane: 1. Hobaneng tlhahlobo ea ONPG e ka sebelisoa ho khetholla ho lieha ho belisoa ha lactose?2. Ke hobane'ng ha maemo a naha a bolela hore hoa hlokahala ho sebelisa 3% sodium chloride trisaccharide iron (kapa trisaccharide iron) bakeng sa tlhahlobo ea ONPG?3. Bakeng sa Vibrio parahaemolyticus, ha u etsa tlhahlobo ea OPNG, ke hobane'ng ha toluene e lokela ho eketsoa ho latela maemo?Mosebetsi ke ofe?
Khamphani ea rona e hlahlobile lintlha tse ngata, ea li akaretsa, 'me ea li arolelana le uena ka tlase:
Molao-motheo: Lebitso la Sechaena la ONPG ke o-nitrobenzene-β-D-galactopyranoside.ONPG e ka tšeloa ka hydrolyzed hore e be galactose le yellow o-nitrophenol (ONP) ka β-galactosidase, kahoo ts'ebetso ea β-galactosidase e ka bonoa ka phetoho ea mebala ea setso.
Lactose ke tsoekere eo likokoana-hloko tse ngata li hlokang ho e lemoha.Metabolism ea eona e hloka li-enzyme tse peli, e 'ngoe ke cell permease, lactose e kena liseleng tlas'a ketso ea permease;e 'ngoe ke β-galactosidase, e etsang hydrolyze lactose hore e be galactose.Lactose le tsoekere.β-Galactosidase le eona e ka sebetsa ka kotloloho ho ONPG ho e etsa hydrolyze hore e be galactose le yellow o-nitrophenol (ONP).E ka etsoa ka 24h, esita le ka lactose e liehang ho fermenters.Ka hona, e hlalosa liphello tsa ho khetha setso sa 1 ho tloha ho agar slant le ho e kenya ka selikalikoe se feletseng ho 36 ° C bakeng sa 1-3h le 24h.Haeba β-galactosidase e hlahisoa, e tla fetoha mosehla ka 1-3h, haeba ho se na enzyme e joalo, e ke ke ea fetola 'mala ka 24h.
Ho ea ka li-enzyme tse peli tse ka holimo, likokoana-hloko li ka aroloa ka lihlopha tse latelang:
1 lactose-fermenting (lihora tse 18-24) libaktheria tse nang le permease le β-galactosidase P + G +;
2 Li-fermenters tsa lactose tse liehang (tse nkang nako e telele ho feta lihora tse 24) li haelloa ke permease empa li na le galactosidase: P- G+.
3 Li-fermenters tse se nang lactose tse haelloang ke permease le galactosidase: P- G-.
Teko ea ONPG e ka sebelisoa ho khetholla libaktheria tsa lactose-lag-fermenting (P-G+) ho libaktheria tsa lactose tse sa beliseng (PG-), joalo ka:
1 Hlalosa li-fermenters tsa morao-rao tsa lactose (P- G+) ho tloha ho li-non-lactose fermenters (P- G-).
(a) Citrobacter (+) le Salmonella arizonae (+) ho tloha Salmonella (-).
(b) Escherichia coli (+) ho tsoa ho Shigella sonnei (-).
Hobaneng ha tlhahlobo ea ONPG e entsoe ho sebelisoa moetlo oa bosiu bo le bong ho 3% sodium chloride ferric trisaccharide (iron trisaccharide)?Khampani ea rona e buisane le boitsebiso bo bongata, empa ha ho na polelo e hlakileng.Ke feela webosaeteng ea FDA, ho ngoliloe hore "Litloaelo tse kenyellelitsoeng ho lekoa holim'a li-agar slants tse tharo tsa tsoekere ebe li e kenya lihora tse 18 ho 37 ° C (kapa mocheso o mong o loketseng, ha ho hlokahala) . Nutrient (kapa tse ling) agar slants tse nang le 1.0 % lactose le eona e ka sebelisoa.e bolela: libaktheria tsa teko li ne li kenngoa ka har'a tšepe ea tšepe ea trisaccharide 'me li hōlile ka 37 ° C bakeng sa 18h.Li-agar slants (kapa tse ling) tse nang le 1% lactose le tsona lia amoheleha.Ka hona, ho nahanoa hore motsoako oa tšepe oa trisaccharide o na le lactose.Kamora ho hola ka bosiu bo le bong, baktheria e hlahisitse β-galactosidase e sebetsang hantle.Ka ho sebelisa libaktheria tse joalo, ONPG e ka bola ka β-galactosidase kapele.Ketsahalo ea liteko e potlakile ebile e bonahala hantle.Ho feta moo, ho eketsa toluene le ho hlapa metsi bakeng sa metsotso e 5 kaofela ke molemong oa ho lokolla β-galactosidase ka botlalo.
