Pale ea mofuta oa β-glucosidase, bgl1G5, e entsoe ho tsoa ho Phialophora sp.G5 mme e hlalositsoe ka katleho ho Pichia pastoris.Tlhahlobo ea tatelano e bonts'itse hore lefutso le na le foreimi ea ho bala e bulehileng ea 1,431-bp e kenyang protheine ea 476 amino acid.Tatelano e entsoeng ea amino acid ea bgl1G5 e bonts'itse boitsebiso bo phahameng ba 85% e nang le β-glucosidase e tsoang ho Humicola grisea ea glycoside hydrolase family 1. Ha e bapisoa le lihlopha tse ling tsa fungal, Bgl1G5 e bontšitse ts'ebetso e ts'oanang e nepahetseng ho pH 6.0 le 50 ° C e ne e le 50 ° C. ka pH 5.0–9.0.Ho feta moo, Bgl1G5 e bonts'itse thermostability e ntle ho 50 ° C (6 h halofo ea bophelo) le ts'ebetso e ikhethileng e phahameng (54.9 U mg–1).Litekanyetso tsa K m le V max ho ea ho p-nitrophenyl β-D-glucopyranoside (pNPG) e ne e le 0.33 mM le 103.1 μmol min–1 mg–1, ka ho latellana.Teko e ikhethang ea substrate e bonts'itse hore Bgl1G5 e sebetsa haholo khahlano le pNPG, e fokola ho p-nitrophenyl β-D-cellobioside (pNPC) le p-nitrophenyl-β-D-galactopyranoside (ONPG), 'me e ne e se na ts'ebetso ho cellobiose.
