Lihlahisoa

Tšebeliso e ntlafetseng ea naetrojene likhomong e bohlokoa molemong oa ho boloka tlhahiso e tsitsitseng ea likhomo.Kaha li-purines le pyrimidines (PP) li etsa karolo ea bohlokoa ea rumen nitrogen, kutloisiso e ntlafetseng ea ho monya le ho kenella ha metabolism ea PP ho bohlokoa.Mosebetsi oa hona joale o hlalosa nts'etsopele le netefatso ea mokhoa o hlokolosi le o ikhethileng bakeng sa qeto e ts'oanang ea li-purines tse 20 (adenine, guanine, guanosine, inosine, 2'-deoxyguanosine, 2'-deoxyinosine, xanthine, hypoxanthine), pyrimidines (cytosine, thymine, uracil, cytidine, uridine, thymidine, 2'-deoxyuridine), le lihlahisoa tsa tsona tse senyang (uric acid, allantoin, β-alanine, β-ureidopropionic acid, β-aminoisobutyric acid) ka plasma ea mali ea likhomo tsa lebese.Mokhoa o phahameng oa ts'ebetso ea mokelikeli oa chromatography o kopantsoeng le electrospray ionization tandem mass spectrometry (LC-MS/MS) o ne o kopantsoe le litekanyetso tsa calibration tse tsamaellanang le matrix le metsoako e tsitsitseng e ngotsoeng ka isotopically.Tlhahlobo ea palo e ile ea etelloa pele ke mokhoa o mocha oa phekolo ea pele ho phekolo o nang le pula ea ethanol, ho hloekisa, ho fetoha mouoane le ho tsosolosoa.Mekhahlelo ea karohano le ho lemoha nakong ea tlhahlobo ea LC-MS/MS e ile ea batlisisoa.Ho netefalitsoe hore ho sebelisa mohlala oa ho lekanya li-log ho e-na le mohlala oa linear calibration ho felletse ka tlase ho CV% mme khaello ea tlhahlobo e nepahetseng e bonts'itse ho fokotseha ho khotsofatsang.Mokhoa ona o akaretsa mekhahlelo ea mahloriso bakeng sa metabolite ka 'ngoe ho ea ka lisampole tsa sebele, mohlala, guanine: 0.10-5.0 μmol/L, le allantoin: 120-500 μmol/L.CV% bakeng sa mekhahlelo e khethiloeng ea palo e ne e le ka tlase ho 25%.Mokhoa ona o na le ho pheta-pheta hantle (CV%≤25%) le ho nepahala ha lipakeng (CV%≤25%) le ho hlaphoheloa ho babatsehang (91-107%).Li-metabolite tsohle li bontšitse botsitso ba nako e telele le botsitso bo botle ka har'a li-runs (CV%≤10%).Ho ile ha bonoa likhato tse fapaneng tsa litlamorao tsa matrix ho plasma, moroto le lebese.Boikemisetso ba litlamorao tse amanang le matrix bo senotse hore mokhoa ona o ne o loketse hoo e batlang e le li-metabolite tsohle tsa PP tse hlahlobiloeng ka har'a plasma e nkiloeng ho artery le portal hepatic, hepatic le gastrosplenic veins mme, ntle le mekhelo e fokolang, le bakeng sa mefuta e meng e kang khoho, kolobe, mink, motho le rat.