Lihlahisoa

Li-brucella ke li-etiological agents tsa brucellosis, zoonosis e ajoang lefatšeng ka bophara.Libaktheria tsena ke li-parasite tsa facultative intracellular 'me ka hona li khona ho fetola metabolism ea tsona ho ea libakeng tse ling le tse ka hare ho lisele tse fumanoang nakong ea tšoaetso ea tšoaetso.Leha ho le joalo, karolo ena ea baeloji ea Brucella ha e utloisisoe hantle, 'me limatlafatsi tse fumanehang ka har'a niche ea intracellular ha li tsejoe.Mona, re batlisisa litsela tse bohareng tsa C metabolism e sebelisoang ke Brucella abortus ka ho hlakola putative fructose-1,6-bisphosphatase (fbp le glpX), phosphoenolpyruvate carboxykinase (pckA), pyruvate phosphate dikinase (ppdK), le enzyme ea malic (mae) liphatsa tsa lefutso.Ho gluconeogenic empa eseng mecheng ea litaba e ruileng, kholo ea ΔppdK le Δmae mutants e ile ea senyeha haholo mme kholo ea phetoho e habeli ea Δfbp-ΔglpX e fokotsehile.Ho macrophages, ke liphetoho tsa ΔppdK le Δmae feela tse bonts'itseng ho ata ho fokotsehile, 'me lithuto tse entsoeng ka ΔppdK mutant li netefalitse hore e fihlile sebakeng sa ho ikatisa.Ka mokhoa o ts'oanang, feela liphetoho tsa ΔppdK le Δmae li ile tsa ts'oaroa ke litoeba, tsa pele li ile tsa hlakisoa ke beke ea 10 mme tsa morao-rao li tšoarella nako e telele ho feta libeke tsa 12.Hape re ile ra etsa lipatlisiso ka potoloho ea glyoxylate.Leha ts'ebetso ea motšehetsi oa aceA (isocitrate lyase) e ile ea ntlafatsoa ka mokhoa o ruileng, tšitiso ea aceA ha ea ka ea ba le phello ea in vitro kapa katisong ea macrophages kapa spleens ea toeba.Liphello li fana ka maikutlo a hore B. abortus e hōla ka har'a intracellularly e sebelisa phepelo e fokolang ea tsoekere ea 6-C (le 5-C) e lefshoang ke glutamate 'me mohlomong le li-amino acid tse ling tse kenang potolohong ea Krebs ntle le karolo ea bohlokoa ea glyoxylate shunt.