Lihlahisoa

Selelekela: BSA ke e 'ngoe ea liprotheine tse sebelisoang haholo lilaboratoring tsa biochemical, 'me bohlokoa ba eona bo ka hlokomolohuoa litekong hobane e atile haholo ebile e tloaelehile.Bovine serum albumin (BSA), eo hape e tsejoang e le karolo ea bohlano, ke globulin ka har'a serum ea likhomo e nang le masalla a 583 amino acid, e boima ba molek'hule ea 66.430kDa le ntlha ea isoelectric ea 4.7.BSA e na le mefuta e mengata ea lits'ebetso litekong tsa biochemical, joalo ka Setsi sa Thibelo libakeng tsa bophirima.

Kopo: Bovine Serum Albumin (BSA), e tsejoang hape e le karolo ea bohlano, ke globulin e serum ea likhomo, e nang le masalla a 607 a amino acid, 'me e na le mefuta e mengata ea ts'ebeliso ea liteko tsa biochemical.Bovine serum albumin ka kakaretso e sebelisoa e le stabilizer tharollong ea polokelo le tharollo ea karabelo ea li-enzyme tse thibelang kapa li-enzyme tse fetotsoeng ho tsitsisa ts'ebetso ea enzyme le ho thibela ho bola ha enzyme le ho ipapisa ka tsela e sa khetheheng.

Mosebetsi: BSA hangata e sebelisoa e le mokhoa oa ho tsitsisa tharollong ea polokelo le tharollo ea karabelo ea li-enzyme tse thibelang kapa li-enzyme tse fetotsoeng, hobane li-enzyme tse ling ha li tsitsitse kapa li na le ts'ebetso e tlase maemong a tlase.Kamora ho eketsa BSA, e kanna ea bapala karolo ea "ts'ireletso" kapa "mojari", mme ts'ebetso ea li-enzyme tse ngata e ka ntlafatsoa haholo kamora ho eketsa BSA.Li-enzyme tse sa hlokeng ho eketsoa ha BSA hangata ha li amehe ke tlatsetso ea BSA.Bakeng sa boholo ba DNA ea substrate, BSCA e ka etsa hore tšilo ea lijo e be e phethahetseng haholoanyane, 'me e ka finyella ho itšeha khafetsa.Ho 37 ° C, ha ts'ebetso ea tšilo ea lijo e feta 1 h, BSA e ka etsa hore enzyme e tsitsitse haholoanyane, hobane ka har'a buffer ea karabelo ntle le BSA, li-enzyme tse ngata tsa thibelo li ka phela feela ho 37 ° C bakeng sa 10 ~ 20min kapa nako e khutšoanyane..Ka lehlakoreng le leng, BSA e ka tlama li-ion tsa tšepe le lik'hemik'hale tse ling ka har'a buffer kapa substrate DNA e thibelang tšebetso ea li-endonuclease tse thibelang.

Lisebelisoa: Standard grade bovine serum albumin (BSA, StandardGrade), e ka finyellang litlhoko tsa liteko tse ngata tse tloaelehileng, tse kang immunoblocking agent, cell cell cell (phoofolo ea likokoana-hloko le lisele tsa likokoanyana, joalo-joalo) limatlafatsi tsa setso le likarolo tsa setso, botsitso ba protheine / enzyme. li-reagents le litekanyetso tsa protheine quantification.Diagnostic grade bovine serum albumin (BSA, DiagnosticGrade) e ka fihlela litlhoko tse ngata tsa liteko tse tloaelehileng, tse kang immunoblocking agent, protheine/enzyme stabilizer, diluent, carrier le protheine standard.Ntle le moo, e ka sebelisoa hape bakeng sa liteko tsa immunoassays, setso sa lisele le liteko tsa hybridization tse hlokang kutloisiso e phahameng.