D-Luciferin Cas: 2591-17-5 99% Off-white ho ea mosehla phofo nbsp BEETLE LUCIFERIN
| Nomoro ea lethathamo | XD90248 |
| Lebitso la Sehlahiswa | D-Luciferin |
| CAS | 2591-17-5 |
| Foromo ea limolek'hule | C11H8N2O3S2 |
| Boima ba Molek'hule | 280.323 |
| Lintlha tsa polokelo | -15 ho ea ho -20 ° C |
| Khoutu ea Lekhetho e Kopantsoeng | 29342080 |
Tlhaloso ea Sehlahisoa
| Litaba tsa Metsi | Max.2.0% |
| Moferefere | Boholo ba 2.0 NTU |
| Potoloho e khethehileng ea optical | -36 ho ea ho -32 |
| Ponahalo | Phofo e tšoeu ho isa ho e mosehla |
| Bohloeki HPLC | Ka tlase ho 99% |
| Molar extinction coefficient | mets 17900 L/(mol cm) |
Selelekela: D-luciferin ke substrate bakeng sa karabelo ea bioluminescence e itšetlehileng ka adenosine triphosphate (ATP).Molao-motheo oa bioluminescence ke hore luciferin e oxidized ke luciferase ka pel'a ATP le oksijene.Mokhoa oa ho arabela lik'hemik'hale o tjena: ATP+D-Luciferin+O2→Oxyluciferin+AMP+PPi+O2+Leseli.
Mokhoa oa ts'ebetso: Mokhoa oa ts'ebetso oa D-luciferin ke hore tlas'a ketso ea ATP le luciferase, luciferin (substrate) e ka oxidized ho ntša leseli.Ha luciferin e feta, palo ea photon e hlahisoang e amana hantle le mahloriso a luciferase.
Kopo: D-luciferin ke substrate bakeng sa liketso tsa bioluminescence tse itšetlehileng ka adenosine triphosphate (ATP).Phello ea bioluminescence ea luciferin / luciferase e atisa ho sebelisoa bakeng sa ho lemoha ATP, metabolites e ka fetoloang ATP (e kang AMP, ADP, cAMP), le li-enzyme tse ka hlahisang ATP (joaloka creatine kinase, joalo-joalo), kahoo karabelo ea bioluminescence e ka sebelisoa.E sebetsa ho lemoha mefuta e mengata ea lintho tse phelang.
Ts'ebetso ea Likokoana-hloko: D-Luciferin (Firefly luciferin) ke substrate e tummeng bakeng sa bioluminescence ka pel'a ATP bakeng sa ho sebelisoa litšoantšong tsa bioluminescence tse thehiloeng ho luciferase le ho hlahloba lisele tse phahameng tsa lisele.
Lithuto tsa in vitro: D-luciferin e sebetsana le luciferase, ATP le oksijene ho ntša leseli, le bonoang ka filimi e bobebe ea lifoto ho bona li-antibodies tse tlanngoeng ke alkaline phosphatase.
Lithuto tsa in vivo: tšebeliso ea li-substrates tsa D-luciferin le li-firefly luciferase li boloka litšebelisano tsa 'mele oa ho itšireletsa mafung ka mokhoa oa immunocompetent mouse o nang le kankere ea mahe a bomme, kaha mananeo a bioluminescence a ameha haholo ho hola ha hlahala ho feta litaelo tsa boima ba' mele.
