PUROMYCIN DIHYDROCHLORIDE Cas: 58-58-2 98% phofo e tšoeu
| Nomoro ea lethathamo | XD90195 |
| Lebitso la Sehlahiswa | Puromycin dihydrochloride |
| CAS | 58-58-2 |
| Foromo ea limolek'hule | C22H31Cl2N7O5 |
| Boima ba Molek'hule | 544.431 |
| Lintlha tsa polokelo | -15 ho ea ho -20 ° C |
| Khoutu ea Lekhetho e Kopantsoeng | 29419000 |
Phapang ea li-fibroblasts tsa orbital ho li-adipocyte tse hōlileng le ho bokellana ha lisele tsa adipose ho bontšitsoe tsoelo-peleng ea Graves' orbitopathy (GO).Autophagy e ameha ho adipogenesis, empa ha ho tsejoe hakaalo ka karolo ea autophagy ho qalisoa le tsoelo-pele ea GO.Sepheo sa thuto ena ke ho batlisisa karolo ea autophagy ho pathogenesis ea GO.Orbital adipose / connective tissue explants ho tsoa ho bakuli ba nang le GO le ho tsoa ho lihlooho tse tloaelehileng, hammoho le li-fibroblasts tse ka thōko tsa orbital, li ile tsa hlahlojoa.Adipogenesis e ile ea hlohlelletsoa ho sebelisa phapang pakeng tsa hydrogen peroxide kapa ntle le eona, 'me autophagy e ile ea sebelisoa ho sebelisoa bafilomycin A1 le Atg5-targeted short hairpin RNA (shRNA).Li-autophagosomes li ile tsa khetholloa ka electron microscopy.Polelo ea liphatsa tsa lefutso tse amanang le autophagy le lintlha tse ngotsoeng tse amanang le adipogenesis li ile tsa hlahlobjoa ke mokhoa oa sebele oa reverse transcription-polymerase chain reaction le/kapa Western blot analysis.Ho bokellana ha marotholi a Lipid ho ile ha hlahlojoa ke Oil Red O staining. Li-vacuoles tsa Autophagic li ne li le ngata ho lisele tsa GO ho feta lisele tse sa GO (p<0.05).Polelo ea liphatsa tsa lefutso tse amanang le autophagy e ne e phahame haholo ho li-tissue tsa GO le liseleng ho feta ho balekane ba bona bao e seng GO, ka ho latellana.Interleukin-1β e eketsehile LC3-II, p62, le protheine ea Atg7 ho lisele tsa GO.Ho bokellana ha Autophagosome ho ile ha bontšoa ka letsatsi la 4 la adipogenesis mme ea fokotseha ka letsatsi la 10, hammoho le sebopeho sa marotholi a lipid.Pontšo ea liprotheine tsa LC3 le p62 e ile ea eketseha ka hare ho lihora tse 48 tsa phapang 'me ea fokotseha butle-butle ho tloha letsatsing la 4 ho ea ho la 10. Phekolo ea Bafilomycin A1 le Atg5 knockdown ka shRNA e thibela ho bokellana ha marotholi a lipid le pontšo e hatelitsoeng ea matšoao a adipogenic. lisele le lisele tse se nang GO, tse fanang ka maikutlo a hore autophagy e bapala karolo ho GO pathogenesis.Autophagy manipulation e kanna ea ba sepheo sa kalafo sa GO.
4-Nitrophenyl-N-acetyl-β- D-glucosaminide ke substrate e molemo bakeng sa tlhahlobo ea colorimetric e potlakileng ea mosebetsi oa N-acetyl-b-glucosaminidase morong oa motho.Chromogenic β-Glucosaminidase substrate e fana ka tharollo e mosehla ha e petsoha, e sebelisoang haholo-holo tomoso le hlobo.
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