PMSF Cas: 329-98-6 98.0% phofo e tšoeu ea crystalline Phenylmethanesulfonyl fluoride (PMSF)
| Nomoro ea lethathamo | XD90250 |
| Lebitso la Sehlahiswa | Phenylmethanesulfonyl fluoride (PMSF) |
| CAS | 329-98-6 |
| Foromo ea limolek'hule | C7H7FO2S |
| Boima ba Molek'hule | 174.1927 |
| Lintlha tsa polokelo | Ambient |
| Khoutu ea Lekhetho e Kopantsoeng | 29049900 |
Tlhaloso ea Sehlahisoa
| Tlhahlobo | ≥98.0% HPLC |
| Ponahalo | phofo e tšoeu ea kristale |
PMSF ke serine/cysteine protease inhibitor e sa fetoheng e sebelisoang hangata ho lokiseng li-lysate tsa lisele.
Lithuto tsa in vitro: PMSF (2 mM) e thibela carbachol-stimulated inositol phosphate ho bokella ka 15% -19% feela ka pel'a Li +.Thibelo ea phetoho ea phosphoinositide ke PMSF e bakoa ke mohato o le mong kapa ho feta ka mor'a ho senyeha ha phosphoinositides [1].PMSF e thibela acylation ea masala a inositol a GPI intermediates maling a T. brucei.PMSF e thibela tlhahiso ea glycolipid C empa eseng ho lokisoa ha acid e mafura ka har'a vitro.PMSF e thibela ho eketsoa ha GPI acylation le ethanolamine phosphatase ho procyclic trypanosomes, empa eseng ka lisele tsa Hela [2].
Lithuto tsa in vivo: PMSF (0.1 mL / 10 g b.wt, ip) e hlahisitse antinociception, joalokaha ho bontšoa ke keketseho e arabelang lethal dose ho% MPE tlhahlobo ea latency ea mohatla, empa e hlōlehile ho hlahisa thibelo e hlakileng ea motlakase e arabelang lethal dose.Litoeba tse amohetseng ente ea intraperitoneal ea PMSF li bonts'itse litlamorao tsa cannabinoid ho kenyelletsa antinociception, hypothermia le ho se sisinyehe ka litekanyetso tsa ED50 tsa 86, 224 le 206 mg / kg, ka ho latellana.PMSF (30 mg/kg) pretreatment e ntlafatsa litlamorao tsa anandamide ho likarabo tsa mohatla (antinociception), ts'ebetso ea locomotor le ho tsamaea ka 5-fold, 10-fold le 8-fold, ka ho latellana[3].
Liteko tsa liphoofolo: Litoeba tse tona tsa ICR tse boima ba 18 ho isa ho 25 g li sebelisitsoe tekong.PMSF e ile ea qhibiliha ka oli ea sesame 'me ea kenngoa ka har'a peritoneally ka bongata ba 0.1 mL/10 g b.wt.Kamehla fana ka PMSF metsotso e 10 pele ho anandamide ka methapo kapa ente ea koloi.Litoeba li ile tsa tloaeleha ho kena phaposing ea tlhahlobo bosiu bo le bong ntle le tšitiso ea lijo kapa metsi.Ka mor'a tsamaiso ea methapo ea anandamide kapa ea koloi, phoofolo e 'ngoe le e' ngoe e ile ea hlahlojoa ka tsela e latelang: Metsotso ea 5 bakeng sa likarabo tsa mohatla-flick latency (antinociceptive) le 5 ho metsotso ea 15 bakeng sa mosebetsi o ikemetseng (motor);kapa metsotso e 5 bakeng sa mocheso oa mantlha (rectal) le ring Immobilization (catalepsy) metsotso e 5 ho isa ho e 10.
