Lihlahisoa

4-Methylumbelliferyl β-D-glucopyranoside e 'nile ea sebelisoa e le substrate: · in glucosylceramidase β enzyme assay assay in lysosome-enriched fractions from primary hippocampal neurons · in β-glucosidase assay during yeast fermentation · to assay glusebrosi-1GB ts'ebetso ho macrophage cell line (RAW)

4-Methylumbelliferyl β-D-glucopyranoside ke substrate ea maiketsetso ea enzymatic bakeng sa glycosidase.E sebelisoa e le substrate bakeng sa β-glucosidase ho tsoa ho enterococci.

Re tlaleha hore bafani ba bonolo ba glycoside ba ka fetola haholo ho lekanngoa ha glycosyltransferase-catalyzed reactions, ho fetola sebopeho sa NDP-tsoekere ho tloha ho endothermic ho ea ho exothermic process.Ho bonts'a ts'ebeliso ea mokhoa ona oa ho ikamahanya le maemo a thermodynamic, re totobatsa motsoako oa glycosyltransferase-catalyzed oa 22 nucleotide ea tsoekere e tsoang ho bafani ba tsoekere e monko o monate, hammoho le sebopeho se ts'oanang sa li-nucleotide tsa tsoekere e le tšusumetso maemong a glycosyltransferase-catalyzed reactions. molek'hule e nyane ea glycodiversification.Bafani bana ba monko o monate ba boetse ba nolofalitse tlhahlobo e akaretsang ea colorimetric bakeng sa glycosyltransfer, e sebetsang ho sibolloeng ha lithethefatsi, boenjiniere ba protheine le lipatlisiso tse ling tsa mantlha tse itšetlehileng ka nucleotide ea tsoekere.Phuputso ena e phephetsa ka ho toba khopolo e akaretsang ea hore li-NDP-tsoekere ke bafani ba tsoekere ba 'matla a phahameng' ha ba tlosoa sebakeng sa bona sa tlhaho sa baeloji.