Lihlahisoa

Monogalactopyranosides ea fluorescein le fluorescein methyl ester: synthesis, enzymatic hydrolysis ka biotnylated β-galactosidase, le boikemisetso ba ho fetolela coefficient ea phallo.

Fluorescein monoglycosides (d-galactopyranoside (FMG) le d-glucopyranoside) le methyl ester (MFMG) ea tsona e lokisitsoe ho tsoa ho acetobromoglucose/galactose le fluorescein methyl ester ka lihlahisoa tse ntle.Ho entsoe liteko tsa enzymatic hydrolysis (ho sebelisa biotinylated β-galactosidase) ea lintho tse nkiloeng ho galacto 'me ho balletsoe litekanyetso tsa kinetic.Keketseho ea makhetlo a 15-20 ea matla a fluorescence e bonoa nakong ea hydrolysis.Keketseho e lekanang ea fluorescence e hlokometsoe ka nako e khuts'oane le khatello e tlase ea substrate, e leng ho etsang hore metsoako ena e be li-probe tse sebetsang hantle bakeng sa galactosidase.Bongata ba boleng ba Michaelis–Menten constant (Km) bakeng sa MFMG bo phahame ho feta ba FMG bo fanang ka maikutlo a hore ho ka ba le phetoho e ka bang teng ya phetohelo ya substrate ya fluorogenic.Km ea boleng ba biotinylated β-Gal e nang le FMG e tlase ho feta ea enzyme ea tlhaho.Tlhaloso ena e bontša kamano e phahameng ea substrate ea enzyme ea biotinylated ha e bapisoa le enzyme ea tlhaho.Li-coefficients tsa phetisetso ea phetolelo li lekantsoe, bakeng sa li-substrates tsa fluorogenic le lihlahisoa ka bobeli, ho sebelisoa spectroscopy ea fluorescence correlation.Li-coefficients tsa phetisetso ea phetolelo bakeng sa li-substrates tsa fluorogenic le lihlahisoa tsa enzymatic hydrolysis li lekantsoe hore li tšoane, ka mefuta ea 3.5–4.5 × 10−10 m2 s−1.Ka hona, ntlafatso kapa tšitiso ea kinetics ea enzymatic ka lebaka la phapang ea motsamao oa phetolelo ea substrate le sehlahisoa ha e bonahale.