Lihlahisoa

Re kile ra kopana le lits'ebetso tsa taolo moo dihydrotestosterone (DHT) e ileng ea fana ka phello ea eona ea ho thibela ho hatelloa ha liphatsa tsa lefutso tsa parathyroid hormone (PTHrP) ka estrogen receptor (ER) α, empa eseng androgen receptor (AR), liseleng tsa MCF-7 tsa mofetše oa matsoele. .Mona, re ile ra batlisisa hore na tšebelisano e joalo ea aberrant ligand-nuclear receptor (NR) e teng liseleng tsa LNCaP tsa mofetše oa senya.Ntlha ea pele, re tiisitse hore lisele tsa LNCaP li bontšitse palo e kholo ea AR maemong a sa tsotelleng a ERα / β kapa progesterone receptor.Ka bobeli khatello ea PTHrP le ts'ebetso ea liphatsa tsa lefutso tsa prostate-specific antigen li ile tsa hlokomeloa ka mor'a tsamaiso e ikemetseng ea 17β-estradiol (E2), DHT, kapa R5020.Tumellanong le mohopolo oa hore LNCaP AR e lahlehetsoe ke sebopeho sa eona sa ligand ka lebaka la phetoho (Thr-Ala877), liteko tsa siRNA tse shebaneng le NR li senotse hore AR e ne e laola karolo ea mokena-lipakeng oa taolo e arolelanoang e itšetlehileng ka li-hormone, e neng e amana ka linako tsohle. ka nuclear translocatio n ea AR ena e fetotsoeng.Tlhahlobo ea Microarray ea taolo ea liphatsa tsa lefutso ka DHT, E2, kapa R5020 e senotse hore karolo e fetang halofo ea liphatsa tsa lefutso tse tlase ho AR (Thr-Ala877) li ne li fetana ka har'a lisele tsa LNCaP.Ho khahlisang haholo, re ile ra hlokomela hore AR (mofuta oa hlaha [wt]) le AR (Thr-Ala877) li ne li ikarabella ka ho lekana bakeng sa litšebelisano tsa E2-AR.Liteko tsa microscopy tsa Fluorescence li bonts'itse hore EGFP-AR (wt) le EGFP-AR (Thr-Ala877) ka bobeli li ne li le sebakeng sa khubu ka mor'a kalafo ea E2 kapa DHT.Ho feta moo, litlhahlobo tsa baqolotsi ba litaba li senoletse hore lisele tse ling tsa mofetše li bonts'itse matšoao a E2-AR (wt) a tšoanang le a liseleng tsa LNCaP.Mona re fana ka maikutlo a ho ba teng ha litšebelisano tse ferekaneng pakeng tsa wt AR le E2 liseleng tse ling tsa mofetše tse sa utloeleng lihomone.