Proteinase K bakeng sa albamo ea tritirachium Cas: 39450-01-6 99% phofo e tšoeu
Nomoro ea lethathamo | XD90434 |
Lebitso la Sehlahiswa | Proteinase K bakeng sa albamo ea tritirachium |
CAS | 39450-01-6 |
Foromo ea limolek'hule | Setšoantšo sa C29H27N2O12P |
Boima ba Molek'hule | 626.50468 |
Lintlha tsa polokelo | -15 ho ea ho -20 ° C |
Khoutu ea Lekhetho e Kopantsoeng | 35079090 |
Tlhaloso ea Sehlahisoa
Matla a Khoheli a khethehileng | 40un / mg protheine min |
Ts'ebetso ea enzyme | 30un / mg min boima bo omileng |
Ponahalo | Phofo e tšoeu |
Tlhahlobo | ≥99% |
Lithuto tsa Genome-wide chromatin immunoprecipitation (ChIP) li tlisitse temohisiso e kholo mabapi le genomic localization ea liprotheine tse amanang le chromatin le liphetoho tsa histone.Palo e kholo ea data e hlahisoang ke litlhahlobo tsena, leha ho le joalo, e hloka mekhoa e lumellang ho netefatsoa ka potlako le tlhahlobo ea bohlokoa ba baeloji.Ho feta moo, ho ntse ho e-na le liprotheine le lipehelo tsa phetoho tseo ho leng thata ho li lemoha ho sebelisa mekhoa e tloaelehileng ea ChIP.Ho rarolla mathata ana, re thehile mokhoa oa hang-hang oa chromatin immunoprecipitation oo re o bitsang ZipChip.ZipChip e fokotsa nako haholo mme e eketsa kutlo e lumellang tlhahlobo e potlakileng ea li-loci tse ngata.Mona re hlalosa kamoo ZipChIP e nolofalletsang ho lemoha liphetoho tsa histone (H3K4 mono- le trimethylation) le li-yeast histone demethylases, Jhd2 le Rph1, tseo pele li neng li le thata ho li lemoha ho sebelisa mekhoa e tloaelehileng.Ho feta moo, re bonts'a ho feto-fetoha ha ZipChIP ka ho sekaseka ntlafatso ea histone deacet ylase Sir2 ho heterochromatin e tomoso le ho ntlafatsa chromatin remodeler, PICKLE, ho euchromatin ho Arabidopsis thaliana.