Proteinase K Cas: 39450-01-6 phofo e tšoeu
| Nomoro ea lethathamo | XD90191 |
| Lebitso la Sehlahiswa | Proteinase K |
| CAS | 39450-01-6 |
| Foromo ea limolek'hule | Setšoantšo sa C29H27N2O12P |
| Boima ba Molek'hule | 626.50 |
| Lintlha tsa polokelo | -15 ho ea ho -20 ° C |
| Khoutu ea Lekhetho e Kopantsoeng | 35079090 |
Tlhaloso ea Sehlahisoa
| Nomoro ea lethathamo | XD90191 |
| Lebitso la Sehlahiswa | Proteinase K |
| CAS | 39450-01-6 |
| Foromo ea limolek'hule | Setšoantšo sa C29H27N2O12P |
| Boima ba Molek'hule | 626.50 |
| Lintlha tsa polokelo | -15 ho ea ho -20 ° C |
| Khoutu ea Lekhetho e Kopantsoeng | 35079090 |
Liphoofolo tse ngata tse nang le li-chromosome tsa thobalano tsa X le Y, mekhoa e pharaletseng ea chromosome e sebelisoa ho leka-lekanya polelo ea X e amanang le liphatsa tsa lefutso ho banna le basali.Ho fofa ea Drosophila melanogaster, mokhoa oa matšeliso a litekanyetso le ona hangata o fetela ho li-transgenes tse amanang le X.Ho entsoe mela e fetang 70 ea mofuta oa linku tsa Australia Lucilia cuprina e le karolo ea boiteko ba ho hlahisa mefuta ea banna feela bakeng sa lenaneo la taolo ea liphatsa tsa lefutso tsa likokoanyana tsena tse kholo tsa linku.Mela eohle e na le mofuta oa protheine ea fluorescent e hlahisoang ka mokhoa o hlakileng.Meleng eohle e 12 e hokahantsoeng, li-larvae tse tšehali li bontša fluorescence e khanyang ho feta li-larvae tse tona, ho fana ka maikutlo a hore mofuta oa liphatsa tsa lefutso ha o lefelle litekanyetso.Sena se netefalitsoe ke palo ea RT-PCR bakeng sa mela e khethiloeng.Ho fumana hore na liphatsa tsa lefutso tsa endogenous X-linked li lefelloa litekanyetso, re khethile liphatsa tsa lefutso tse 8 tseo e leng liphatsa tsa lefutso tse ho chromosome ea bone ho D. melanogaster.Bopaki ba morao tjena bo fana ka maikutlo a hore D. melanogaster four chromosome, kapa Muller element F, ke X chromosome ea baholo-holo ho Diptera e khutletseng ho autosome ho mefuta ea Drosophila.Re bontša ka palo ea PCR ea DNA ea banna le basali hore mefuta e 6 ho e 8 ea sehlopha sa F e lula ho X chromosome ho L. cuprina.Liphatsa tse ling tse peli tsa Muller element F li fumanoe e le autosomal ho L. cuprina, athe mefuta e 'meli ea Muller element B e ile ea fumanoa sebakeng se le seng sa X chromosome le L. cuprina orthologs tsa D. melanogaster Ephrin le gawky gene.Re fumana hore L. cuprina X liphatsa tsa lefutso tsa chromosome li hlahisoa ka ho lekana ho banna le basali (ke hore, litekanyetso li lefshoa ka botlalo).Kahoo, ho fapana le Drosophila, ho bonahala eka tsamaiso ea matšeliso ea litekanyetso tsa Lucilia e totobetse bakeng sa liphatsa tsa lefutso tsa X chromosome 'me e ke ke ea kopanngoa le li-transgene tse sa tsoa fihla.
