Lihlahisoa

Protein phosphorylation e laola mesebetsi e fapaneng ea lisele mme e bapala karolo ea bohlokoa ntlafatsong ea pele ea limela.Ho tlatselletsa le ho holisa lipatlisisong tse fetileng tsa protheine phosphorylation lipeong tsa Arabidopsis re sebelisitse mokhoa o mong o kopanyang ho ntšoa ha protheine tlas'a maemo a sa tsitsang le ntlafatso ea metal-ion affinity chromatography (IMAC) e sa sebetseng ea phosphoprotein ho li-extracts tse senyehileng tsa Rubisco, tse lateloang ke boitsebahatso. ho sebelisa gel electrophoresis ea mahlakore a mabeli (2-DE) le mokelikeli oa chromatography-tandem mass spectrometry (LC-MS/MS).Ho chesoa ha li-trypsin ka gel le tlhahlobo ea matheba a li-gel a khethiloeng a bonts'itseng li-peptide tse 144 tsa phosphorylated le masalla, tseo ho tsona ho fumanoeng li-phosphopeptides tse 18 feela le li-phosphosite tse 8 ho PhosPhat 4.0 le P3DB Arabidopsis thaliana phosphorylation databases.Ho feta halofo ea li-phosphoprotein tse 82 tse khethiloeng li ne li ameha metabolism ea lik'habohaedreite, photosynthesis / respiration kapa mechani sms ea oxidative stress response.Ntlafatso ea phosphoprotein e sa fetoheng pele ho 2-DE le LC-MS/MS e bonahala e ntlafatsa ho fumanoa ha masala a phosphorylated threonine le tyrosine ha a bapisoa le mekhoa e sebelisang ntlafatso ea boemo ba peptide, ho fana ka maikutlo a hore mekhoa ena e 'meli e batla e tlatselletsana mabapi le tšireletso ea sebaka sa phosphorylation.Ho bapisa liphetho bakeng sa lipeo tse nyane le tse fumanoeng pele bakeng sa makhasi a holileng a Arabidopsis a bonts'a liprotheine tse hlano tse nang le phosphorylated ka mokhoa o fapaneng lithong tsena, ho bonts'a bokhoni ba mokhoa ona oa ho batlisisa matla a protheine phosphorylation nakong ea kholo ea semela.