L-Lactate dehydrogenase CAS: 9001-60-9
| Nomoro ea lethathamo | XD90395 |
| Lebitso la Sehlahiswa | L-Lactate dehydrogenase |
| CAS | 9001-60-9 |
| Foromo ea limolek'hule | - |
| Boima ba Molek'hule | - |
| Khoutu ea Lekhetho e Kopantsoeng | 35079090 |
Tlhaloso ea Sehlahisoa
| Ponahalo | Phofo e tšoeu |
Tlhahlobo ea liketsahalo tsa proteome-wide phosphorylation e ntse e le phephetso e kholo ea tlhahlobo ka lebaka la ho rarahana ho hoholo ha marang-rang a protheine phosphorylation.Mosebetsing ona, re lekola ho tlatsana ha Lys-N, Lys-C, le trypsin mabapi le bokhoni ba bona ba ho kenya letsoho tlhahlobisong ea lefats'e ea phosphoproteome.Phetolelo e hloekisitsoeng ea phapanyetsano e matla ea cation e tlaase ea pH e ile ea sebelisoa ho arola ka katleho N-terminally acetylated, phosphorylated, le li-peptide tse sa fetoloang.Kakaretso ea li-phosphopeptide tse sa hlokahaleng tsa 5036 li ne li ka tsejoa ka tekanyo ea bohata ea ho sibolloa ea <1% ho tloha ho 1 mg ea protheine e sebelisang motsoako oa li-enzyme tse tharo.Lintlha tsa rona li senoletse hore ho kopana pakeng tsa lisebelisoa tsa data tsa phosphopeptide tse hlahisitsoeng ka li-protease tse fapaneng li ne li le ka thōko, athe ho kopana pakeng tsa lisebelisoa tse peli tse tšoanang tsa tryptic ho fumanoe bonyane makhetlo a 4 a phahameng.Ka tsela ena, ts'ebeliso e ts'oanang ea Lys-N le trypsin e nolofalelitse keketseho ea 72% palong ea li-phosphopeptides tse fumanoeng ha li bapisoa le d le trypsin feela, athe teko ea ho etsa likopi tsa trypsin e lebisitse ho keketseho ea 25%.Kahoo, ha re shebana feela le data ea trypsin le Lys-N, re khethile li-phosphopeptides tse 4671 tse sa hlokeng letho.Litlhahlobo tse ling tsa libaka tse fumanoeng li bonts'itse hore li-data tsa Lys-N le trypsin li ruisitsoe ka mefuta e fapaneng haholo ea phosphorylation motifs, e leng bopaki bo tsoelang pele ba hore mekhoa ea multiprotease e bohlokoa haholo litlhahlobong tsa phosphoproteome.
