Lihlahisoa

Re ile ra hlahisa mokhoa o mocha oa ho fana ka RNA (siRNA) o kenang o sebelisa mochine oa ternary o nang le polyethyleneimine (PEI) le γ-polyglutamic acid (γ-PGA), e bontšang phello ea ho khutsisa le ho se na cytotoxicity.Li-binary complexes tsa siRNA tse nang le PEI li ne li ka ba 73-102 nm ka boholo ba likaroloana le 45-52 mV ka ζ-potential.Phello e khutsitseng ea li-complexes tsa siRNA / PEI e eketsehile ka ho eketseha ha PEI, 'me li-complexes tsa siRNA / PEI tse nang le tekanyo ea tefiso e kholo ho feta 16 li bontšitse ho kokobela ha luciferase ka har'a mouse colon carcinoma cell line e lulang e hlalosa luciferase (Colon26 / Luc cell).Leha ho le joalo, cytotoxicity e matla le agglutination ea mali li ile tsa hlokomeloa ho siRNA / Lipofectamine complex le siRNA / PEI16 e rarahaneng.Ho nchafatsa li-complexes tsa cationic ka motsoako oa anionic ho tlalehiloe e le mokhoa o ts'episang oa ho hlola chefo ena.Ka hona re ile ra lokisa li-ternary complexes tsa siRNA tse nang le PEI (tefiso ea tekanyo ea 16) ka ho kenyelletsa γ-PGA ho fokotsa cytotoxicity le ho fana ka siRNA.Joalokaha ho lebeletsoe, cytotoxicity ea ternary complexes e fokotsehile ka keketseho ea γ-PGA content, e leng se ileng sa fokotsa ζ-mokhoa oa li-complexes.Phello e matla ea ho khutsisa e bapisoang le siRNA/Lipofectamine complex e ile ea sibolloa litsing tsa ternary ho kenyelletsa γ-PGA e nang le tefiso ea anionic surface.Kenyelletso e phahameng ea li-ternary complexes ka har'a lisele tsa Colon26 / Luc e netefalitsoe ka microcopy ea fluorescence.Ha e se e fihletse ho putlama ha mofuta o fetisoang ka mokhoa o sa tloaelehang, bokhoni ba ho rarahana ho kopanyelletsa ho putlama ha mofuta oa endogenous oa ho boloka ntlo, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), e ile ea hlahlojoa liseleng tsa B16-F10.The ternary complex (siRNA/PEI16/γ-PGA12 complex) e bontšitse phello e kholo ea GAPDH.Kahoo, re thehile sistimi e sebetsang ea phepelo ea siRNA.