Lihlahisoa

The membrane topology ea colicin E1 channel domain name e ile ea ithutoa ke fluorescence resonance energy transfer (FRET).FRET e kenyelelitse liphatsa tsa lefutso tsa amino acid ea fluorescent (coumarin) e le mofani le masalla a cysteine ​​​​a khethiloeng ka mokhoa o khethiloeng a kopantsoeng le DABMI (4-(dimethylamino) phenylazophenyl-4'-maleimide) joalo ka moamoheli oa FRET.Masala a coumarin a fluorescent a kenyelelitsoe ho protheine ka orthogonal tRNA/aminoacyl-tRNA synthetase pair e ileng ea lumella ho kenngoa ho khethehileng sebakeng leha e le sefe ka har'a sebaka sa mocha oa colicin.Mofuta o mong le o mong o ne o e-na le phetoho ea ho emisa (TAG) bakeng sa kenyelletso ea coumarin le phetoho ea cysteine ​​(TGT) bakeng sa sehokelo sa DABMI.Libaka tse tšeletseng tsa li-interhelical ka har'a li-helices tsa 1-6 li ile tsa khethoa ho sebelisoa tlhahlobo ea FRET bakeng sa linaha tse qhibilihisang le tse nang le membrane.Lintlha tsa FRET li bonts'itse liphetoho tse kholo lipakeng tsa li-helices tsa 3-6 holim'a membrane ea membrane e fanang ka temohisiso e ncha mabapi le sebopeho sa membrane ea sebaka sa mocha.Ka kakaretso, ts'ebetso ea coumarin-DABMI FRET interhelical e fokotsehile holim'a ho tlama ha membrane, ho haha ​​​​mohlala oa sekhele bakeng sa mocha oa colicin.Mohlala oa nakoana bakeng sa boemo bo koetsoeng ba sebaka sa marang-rang se ile sa ntlafatsoa ho ipapisitsoe le data ea FRET ea hajoale le e phatlalalitsoeng pele.Mohlala ona o fana ka tlhahiso ea tlhophiso e chitja ea li-helices 1-7 ka tsela e ts'oanang le oache ho tloha ka lehlakoreng le kantle ho sele le membrane interfacial association ea li-helices 1, 6, 7, le 10 ho potoloha bohareng ba transmembrane hairpin e entsoeng ke li-helices 8 le 9.