4-Nitrophenyl phosphate disodium letsoai 6-hydrate CAS:333338-18-4 e tšoeu ho isa phofo e mosehla
| Nomoro ea lethathamo | XD90511 |
| Lebitso la Sehlahiswa | 4-Nitrophenyl phosphate disodium letsoai le 6-hydrate |
| CAS | 333338-18-4 |
| Foromo ea limolek'hule | C6H16NNa2O12P |
| Boima ba Molek'hule | 371.142 |
| Lintlha tsa polokelo | Ambient |
| Khoutu ea Lekhetho e Kopantsoeng | 29199000 |
Tlhaloso ea Sehlahisoa
| Ponahalo | bosoeu ho isa bosehla bo bosehla |
| Tlhahlobo | 99% |
Ho feta 70% ea li-glioma tsa boemo bo tlaase li na le phetoho ea heterozygous R132H ka gene coding bakeng sa isocitrate dehydrogenase 1 (IDH1).Sena se fa enzyme ka bokhoni bo bocha ba ho fetolela α-ketoglutarate ho 2-hydroxyglutarate, e qetellang e lebisa ho tumorigenesis.Mohloli o moholo oa tlhahiso ea 2-hydroxyglutarate ke glutamine, eo, ka mofetše, hape e leng mohloli oa tricarboxylic acid cycle (TCA) anaplerosis.Mohloli o mong oa anaplerosis ke pyruvate flux ka pyruvate carboxylase (PC), e leng tsela e tloaelehileng ho li-astrocyte tse tloaelehileng.Sepheo sa thuto ena e ne e le ho fumana hore na PC e sebetsa e le mohloli oa TCA anaplerosis liseleng tse feto-fetohang tsa IDH1 moo glutamine e sebelisetsoang tlhahiso ea 2-hydroxyglutarate. Li-astrocyte tse tloaelehileng tsa batho tse sa shoeng li entsoe ho hlalosa heterozygous mutant IDH1 kapa mofuta oa naha oa IDH1 o ile oa hlahlojoa.Ho phalla ha pyruvate ka PC le ka pyruvate dehydrogenase (PDH) ho ile ha etsoa qeto ka ho sebelisa spectroscopy ea makenete ea resonance ho lekola ho ngoloa ha [2-¹³C] e nkiloeng ke glucose ¹³C-labe led glutamate le glutamine.Liteko tsa ts'ebetso, RT-PCR le blotting ea bophirima li ile tsa sebelisoa ho lekola polelo le tšebetso ea li-enzyme tse amehang.Lintlha tsa Cancer Genome Atlas (TCGA) li ile tsa hlahlojoa ho hlahloba polelo ea li-enzyme ho lisampole tsa glioma tsa batho.Sena se ne se amahanngoa le keketseho e kholo ea ts'ebetso ea PC le polelo.Ka nako e ts'oanang, ts'ebetso ea PDH e fokotsehile haholo, e ka 'nang ea e-ba lipakeng tsa ho eketseha haholo ho thibela phosphorylation ea PDH ka PDH kinase 3. Ho lumellana le ho hlokomeloa ka liseleng, tlhahlobo ea lintlha tsa TCGA e bontšitse keketseho e kholo ea polelo ea PC ka lisampole tsa glioma tsa motho tse feto-fetohang tsa IDH ha li bapisoa le tse hlaha- mofuta oa IDH. Liphuputso tsa rona li fana ka maikutlo a hore liphetoho ho PC le PDH e ka 'na ea e-ba karolo ea bohlokoa ea phetoho ea cellular ho phetoho ea IDH1 'me e ka sebetsa e le sepheo sa phekolo.
