dCTP, 2'-Deoxycytidine-5'-triphosphoric acid disodium letsoai Cas:102783-51-7 White Powder
Nomoro ea lethathamo | XD90584 |
Lebitso la Sehlahiswa | dCTP, 2'-Deoxycytidine-5'-triphosphoric acid disodium letsoai |
CAS | 951-78-0 |
Foromo ea limolek'hule | C9H12N2O5 |
Boima ba Molek'hule | 228.20 |
Lintlha tsa polokelo | -20 °C |
Khoutu ea Lekhetho e Kopantsoeng | 29349990 |
Tlhaloso ea Sehlahisoa
Ponahalo | Phofo e Tshweu |
Tlhahlobo | 99% |
Lisele tsa Eukaryotic li na le ho leka-lekana ha metsotsoana ea li-deoxyribonucleoside triphosphates (dNTPs), tse lekaneng metsotso e seng mekae feela ea ho ikatisa ha DNA.Ho haella ha dNTP e le 'ngoe hammoho le bolulo ba dNTP e le 'ngoe ho ka baka ho eketseha ha litekanyetso tsa phetoho, ho lokisoa ha DNA e fosahetseng kapa ho fokotseha ha DNA ea mitochondrial.Hangata li-dNTP li lekanyetsoa ka tlhahlobo ea enzymatic eo ho eona ho kenyelletsoa ha radioactive dATP (kapa radioactive dTTP tekong ea dATP) ho li-oligonucleotide tse khethehileng tsa maiketsetso ka DNA polymerase e lekanang le mahloriso a dNTP e sa tsejoeng.Re fumana hore Klenow DNA polymerase e sebelisoang hangata e ka nkela ribonucleotide e tsamaellanang sebakeng sa dNTP e sa tsejoeng e lebisang maemong a mang ho fetelletseng e kholo ea dNTPs.Hona joale re hlalosa maemo a tlhahlobo bakeng sa dNTP ka 'ngoe e qobang ho kenngoa ha ribonucleotide.Bakeng sa litlhahlobo tsa dTTP le dATP ho lekane ho fokotsa likhahla tsa enzyme ea Klenow le tse ngotsoeng dATP (kapa dTTP);bakeng sa dCTP le dGTP re ile ra tlameha ho nkela enzyme ea Klenow sebaka ka Taq DNA polymerase kapa Thermo Sequenase.Re fana ka maikutlo a hore litlalehong tse ling tsa pejana ho kenyelletsoa ha ribonucleotide ho ka 'na ha baka litekanyetso tse phahameng haholo bakeng sa dGTP le dCTP.