Lihlahisoa

APS-5 ke substrate ea chemiluminescent e thehiloeng ho 9,10-dihydroacridine, e sebelisoang haholo-holo bakeng sa ho lemoha ELISA ea metsoako e nang le alkaline phosphatase AP conjugates;9,10-dihydroacridine ka sebopeho sa APS-5 Sebopeho sa hydroacridine se ka hlahisa leseli ka ho tsoelang pele, ka mokhoa o tsitsitseng le ka katleho tlas'a ketso ea AP, 'me APS-5 e loketse haholo bakeng sa tlhahlobo ea tharollo ea phosphatase le phosphatase e amanang le immunoassay ea enzyme.

E le probe e khanyang, e sebelisoa thutong ea liphatsa tsa lefutso.Karabelo e ngotsoe ka acridan (9,10-dihydroacridine) e le substrate le alkaline phosphatase, e hlahisang chemiluminescence e tsitsitseng e phahameng.E fana ka kutloisiso e phahameng le boiketlo ba tšebeliso ea alkaline phosphatase conjugation nakong ea ho lemoha chemiluminescent.Alkaline phosphatase e fumanoa ka tlase ho 10-19 mol, e phahama ka potlako ho fokotsa nako ea ho lemoha le ho eketsa ho feta, 'me moepa oa curve ea calibration ea linear o reriloe ka logarithmic e lekanang le 1.0.Palo e le 'ngoe kapa ho feta ea enzyme e hlahisa palo e le' ngoe kapa ho feta ea luminescence, luminescence e tsoelang pele-e sa hlokeng nako ea teko.Luminescence intensity e ka baloa ho tloha mokokotlong o hlahisitsoeng oa linear calibration ka nako leha e le efe, 'me liphetho tsa tlhahlobo ha li amehe mocheso oa 22 ° C - 35 ° C, ho fokotsa ho nepahala ho hlokahalang ho laola mocheso.

molemo:

1 Kutlo e phahameng - matšoao a protheine a ka tlase ho 5pg a ka bonoa;

2 Matla a khanyang a phahameng - tlhōrō e khanyang e ka finyelloa ka nako e khutšoanyane;

3 Luminescence e tsoelang pele le e tsitsitseng - ts'ebetso ea eona ea luminescence ha e amehe ke mocheso ka hare ho 25-35 ℃, 'me ha e hloke taolo ea mocheso.

Papiso ea ts'ebeliso ea li-substrates tsa luminescent tse sebelisoang hangata APS-5 (193884-53-6) le AMPPD (122341-56-4)

Ka bobeli APS-5 (193884-53-6) le AMPPD (122341-56-4) ke li-chemiluminescent substrates bakeng sa alkaline phosphatase, 'me ka bobeli li bitsoa li-ultra-high sensitivity reagents, ka hona, li-reagents tsena tse peli li fumanehile hore na ke eng e molemo le ho feta. ke efe e mpe?U ka khetha joang ha u e sebelisa?

Ka lehlakoreng le leng, ha APS-5 e sebelisoa e le karolo ea ho lemoha phosphatase ea alkaline, kutloisiso ea ho lemoha e ka fihla ho 10-19 molar concentration, nako ea nts'etsopele ea mebala e ka fihla sehlohlolong ka metsotsoana e ka bang 10, 'me fluorescence e nka nako e telele. nako.Ntlheng ena, APS 5 e ka boleloa e na le thepa e ntle haholo.Leha ho le joalo, APS-5 e lekanyelitsoe mochesong oa ts'ebeliso, 'me mocheso oa eona o fanang ka mebala o ka tlase ho 20'C.Ka lehlakoreng le leng, ha AMPPD e sebelisoa e le substrate ea ho lemoha bakeng sa alkaline phosphatase, kutloisiso ea ho lemoha e boetse e bitsoa ultra-high sensitivity, empa e ke ke ea fihla boemong ba APS 5. Nako ea nts'etsopele ea mebala e fihla tlhōrōng e kholo ka metsotsoana e ka bang 30. , 'me nako ea fluorescence e telele.AMPPD e ntle haholo linthong tse kaholimo, empa ha e le hantle e tlase ho APS5, empa mocheso oa eona oa ho fana ka mebala ha o thata joalo ka APS5, mme mocheso oa polokelo oa 2--8C o bonolo ho feta APS-5.Ka kakaretso, le hoja APS-5 e feta AMPPD ka ho nepahala ha ho fumanoa, e lahleheloa ke botsitso.Tsela ea ho e khetha e itšetlehile ka litlhoko tse khethehileng tsa ho lemoha.

1. Kenyelletso ea APS-5 (193884-53-6):

Lebitso la Senyesemane: APS-5

Nomoro ea CAS: 193884-53-6

Foromo ea molek'hule: C21H15ClNO4PS Na2

Boima ba molek'hule: 489.819

Ponahalo: phofo e tiileng e bosoeu bo bosoeu

Litaba: ≥98%

Litlhaloso: 1g, 5g, 10g

Maemo a polokelo: -20 ℃ ho boloka

(4-Chlorophenylmercapto) (10-methyl-9,10-dihydroacridine methylene) letsoai la phosphate disodium, le bitsoang APS-5, ke reagent ea chemiluminescent 'me e sebelisoa e le substrate ea chemiluminescent.E fana ka kutloisiso e phahameng le boiketlo ba tšebeliso ea alkaline phosphatase conjugation nakong ea ho lemoha chemiluminescent.Karabelo e ngotsoe ka acridan (9,10-dihydroacridine) e le substrate le alkaline phosphatase, e hlahisang chemiluminescence e tsitsitseng e phahameng.APS-5 ke sesebelisoa se nepahetseng bakeng sa qeto ea mosebetsi oa phosphatase ka litlhahlobo tsa tharollo le li-immunoassays tse amanang le phosphatase ea enzyme.Joaloka substrate e khanyang, APS-5 e na le kutloisiso e phahameng ea ho lemoha le nako e khutšoanyane ea karabelo.

2. Kenyelletso e qaqileng ea AMPPD (122341-56-4)

Lebitso la Senyesemane: AMPPD

Nomoro ea CAS: 122341-56-4

Mokhoa oa molek'hule: C18H23O7P

Boima ba molek'hule: 382.344781

Ponahalo: phofo e tšoeu

Bohloeki: ≥97%

Litlhaloso: 1g, 5g, 10g

Maemo a polokelo: -20 ℃ ho boloka

Litlhokomelo: E tlameha ho omella ho qoba ho qhibiliha khafetsa le lyophilization