Lihlahisoa

1.Ho batlisisa hore na adenosine diphosphate (ADP) -derived adenosine e ka thibela platelet aggregation, haholo-holo ka pel'a mohanyetsi oa P2Y₁₂, moo liphello tsa ADP ho P2Y₁₂ receptor li ne li tla thibeloa. peptide ka ho bala platelet ka plasma e nang le platelet-rich (PRP) le mali a feletseng ka pel'a ADP le bahanyetsi ba P2Y₁₂ cangrelor, prasugrel active metabolite, le ticagrelor.Ka pel'a mohanyetsi oa P2Y₁₂, preincubation ea PRP e nang le ADP e thibetsoeng aggregation;phello ena e ile ea felisoa ke adenosine deaminase.Ha ho thibelo ea aggregation e etsahetseng maling a felletseng ntle le ha dipyridamole e kentsoe ho thibela ho kenella ha adenosine ho erythrocyte.Liphello tsa ADP ho PRP le mali a feletseng li ile tsa phetoa ka ho sebelisa adenosine 'me li amana ka ho toba le liphetoho tsa cAMP (e hlahlojoa ke vasodilator-stimulated phosphoprotein phosphorylation).Liphetho tsohle li ne li tšoana ho sa tsotellehe hore na P2Y₁₂ ant agonist e sebelisitsoe.Thibelo e etsahala ho PRP empa eseng maling a felletseng ntle le ha ho kenngoa ha adenosine ho thibetsoe.Ha ho le ea mong oa bahanyetsi ba P2Y₁₂ ea ithutoang ea phethileng litlamorao tsa dipyridamole litekong tse entsoeng.

2.ADP e nkoa e le agonist e fokolang ea platelet ka lebaka la likarabo tse fokolang tsa aggregation tseo e li kenyang ka har'a vitro maemong a physiological of extracellular Ca (2+) [(Ca (2+) )) (o) ].Ho theola [Ca(2+) ](o) ka mokhoa o makatsang ho matlafatsa khokahanyo ea ADP-evoked, phello e 'nileng ea amahanngoa le tlhahiso e matlafalitsoeng ea thromboxane A(2).Phuputso ena e ile ea hlahloba karolo ea ectonucleotidases ho [Ca (2+) ] (o) -ho itšetlehile ka ts'ebetso ea platelet.Ho fokotsa [Ca(2+) ](o) ho tloha ho millimolar ho ea ho micromolar ho fetotsoe ADP (10 μmol/l) -e tsositse pokello ea liplatelete ho tloha ho nakoana ho ea ho karabelo e tsitsitseng ho plasma e nang le platelet e ngata le li-suspension tse hlatsoitsoeng.Ho thibela tlhahiso ea thromboxane A(2) ka aspirin ho ne ho se na phello ho sena [Ca(2+) ](o) -ho itšetleha.Thibelo ea tšenyeho ea ADP e felisitse phapang lipakeng tsa maemo a tlase le a fisioloji [Ca(2+) ](o) e bakang kopanyo e matla le e tsitsitseng maemong ao ka bobeli.Litekanyo tsa extracellular ADP li senotse ho fokotseha ho fokotsehileng ho plasma le apyrase-containin g saline ho micromolar bapisoa le millimolar [Ca(2+) ](o) .Joalokaha ho tlalehiloe pejana, moloko oa thromboxane A(2) o ile oa ntlafatsoa ka tlaase ho [Ca(2+) ](o) , leha ho le joalo sena se ne se ikemetse mosebetsing oa ectonucleotidase(.) P2Y receptor antagonists cangrelor le MRS2179 e bontšitse tlhokahalo ea li-receptor tsa P2Y(12) bakeng sa kopano e tsitsitseng ea ADP, e nang le karolo e nyane bakeng sa P2Y(1) .Qetellong, Ca (2+) -e itšetlehileng ka ectonucleotidase mosebetsi ke ntlha e ka sehloohong e khethollang boholo ba platelet aggregation ho ADP 'me e tlameha ho laoloa bakeng sa lithuto tsa ts'ebetso ea P2Y receptor.