4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99%
| Nomoro ea lethathamo | XD900018 |
| Lebitso la Sehlahiswa | 4-Nitrophenyl-alpha-D-galactopyranoside |
| CAS | 7493-95-0 |
| Foromo ea limolek'hule | C12H15NO8 |
| Boima ba Molek'hule | 301.25 |
| Lintlha tsa polokelo | -15 ho ea ho -20 ° C |
| Khoutu ea Lekhetho e Kopantsoeng | 29400000 |
Tlhaloso ea Sehlahisoa
| Ponahalo | E tšoeu ho ea ho Orange ho ea phofo e tala ho kristale |
| Tlhahlobo | 99% |
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) ke substrate ea maiketsetso ea 4-nitrophenyl (pNP) glycopyranoside bakeng sa ho lemoha mosebetsi oa α-galactosidase.Palo ea pNP e lokollotsoeng e ile ea eketseha haholo ha 4-Nitrophenyl α-D-galactopyranoside e sebelisoa e le substrate.
Using4-nitorophenol (pNP) -glycopyranosidesaspseudosubstrates, theamountoflovedpNPho tloha4-Nitrophenylα-D-galactopyranosideka holimo haholo ho feta ho tloha ho thetherpNP-glycopyranosidesatpH4.0haebaha ho ne ho se na mokhoa o 4. nyl (pNP) glycopyranosidesasartificialsubstrates e bontshathatyieldinliberated galactoseresidues from4-Nitrophenylα-D-galactopyranosidemainlyatpH4.0, ke hore,Hlevelwhereyieldthresholde fokotseha ka ho fetisisa.
Substrate ea α-D-galactosidase (α-D-Galactosidase).E tlama ho E. coli lactose protheine carrier.Hare bakeng sa α-D-galactose.
Mokhoa o potlakileng oa ho lekola tšebetso ea lytic ea li-antimicrobial agents khahlano le tomoso le li-fungus e entsoe.Teko e ipapisitse le ho lokolloa ha enzyme ea intracellular, maltase (alpha-glucosidase).Ts'ebetso ea maltase e lokollotsoeng e lekantsoe ka mebala ka tlhahiso ea p-nitrophenol ho tsoa ho p-nitrophenyl-alpha-D-glucopyranoside (PNPG).Ts'ebetso ea lytic ea metsoako e fapaneng ea antimicrobial e ne e lekantsoe khahlanong le lisele tsa tomoso kapa li-spores tse melang tsa li-fungus tsa filamentous.Ts'ebetso ea Lytic e khahlanong le tomoso e ka bonoa ka har'a metsotso e 20 ea ho fuama ka likhato tse 30 tsa C khahlano le Saccharomyces cerevisiae, Candida albicans, le Cryptococcus neoformans.Tšebetso ea Lytic e khahlanong le fungal e hlahile ka mor'a lihora tse 2 tsa ho fuama ka likhato tse 30 tsa C khahlano le likokoana-hloko tse melang tsa Aspergillus niger le Botrytis cinerea.Lisele tsohle tsa tomoso kapa li-fungus ha lia ka tsa hlahisa PNPG e lekaneng nakong ea lihora tse 3 ho likhato tse 30 tsa C ho hlahisa phetoho e bonahalang ea 'mala.Ts'ebetso ea lytic ea li-enzyme (mohlala, Lyticase), lithibela-mafu (mohlala, Amphotericin B), le mofuta oa libaktheria tse hlahisang lithibela-mafu li ile tsa bonoa ho sebelisoa tlhahlobo.Teko ea anti-yeast e fetotsoe ho sebopeho sa 96-well microtiter.Litlhahlobo tsena ka bobeli li fane ka tlhahlobo e potlakileng, e hlokolosi, le e ka hlahisoang hape ea ts'ebetso ea lytic anti-yeast le anti-fungal.
