Lihlahisoa

Moeli oa ITP o leka-lekaneng oa EOF o sebelisitsoe ho bokella li-anion ho tloha lisampole tsa conductivity tse phahameng nakong ea ente ea electrokinetic e tsoelang pele ea sampole.Ka polystyrenesulfonate/poly(diallyldimethylammonium chloride) polyelectrolyte coated capillary, nako eo moeli oa ITP o neng o tsoa ka eona capillary e ka lelefatsoa ka ho leka-lekanya ho tsamaea ha moeli le EOF.Ho sebelisa electrolyte ea bis-tris-propane, moeli oa ITP o ile oa tlosoa ho capillary ka hare ho 7min, ha ho sebelisoa triethanolamine moeli oa ITP o ne o ntse o le 30% ea capillary ka mor'a 2h ea ente.Ho sebelisoa litsamaiso tsena, kutloisiso ea motsoako oa li-organic acid tse bonolo ho 100mM Cl(-) e ile ea ntlafatsoa ka makhetlo a 700-800 ho sebelisoa bis-tris-propane ka ente ea capillary ea sampole le metsotso e 5 ea ente ea electrokinetic ho +28kV. , le 1100-1300-fold ho sebelisa triethanolamine le 60min ea ente ea electrokinetic tlas'a maemo a tšoanang.Bokhoni ba mokhoa o ka sebelisoang ho lisampole tse phahameng tsa conductivity bo bonahalitsoe ka ho bokella capillary eohle e tlatsitsoeng ka moroto o nang le naphthalenedisulfonic acid, ka meeli ea ho fumanoa ka makhetlo a 450 ho feta e ka fihlellehang ka ente e tloaelehileng ea hydrodynamic.