Trinder's reagent e ncha ke ntho e tsoang ho aniline e qhibilihang haholo metsing e sebelisoang haholo litekong tsa tlhahlobo ea mafu le litekong tsa biochemical.E na le melemo e 'maloa ho feta li-reagents tse tloaelehileng tsa chromogenic qetong ea colorimetric ea ts'ebetso ea hydrogen peroxide.Sehlahisoa se secha sa Trinder se tsitsitse ka ho lekaneng ho tsona ka bobeli E ka sebelisoa tharollong kapa ho sisteme ea tlhahlobo ea lipeipi.Boteng ba hydrogen peroxide le peroxidase, reagent e ncha ea Trinder'si e ka sebelisoa hammoho le 4-aminoantipyrine (4-AA) kapa 3 methyl Nakong ea karabelo ea oxidative coupling ea benzothiazole sulfonehydrazone (MBTH), lidae tse tsitsitseng haholo tse pherese kapa tse putsoa. lia thehoa.Motsoako oa molar oa lidae o kopantsoeng le MBTH o phahame ka makhetlo a 1.5-2 ho feta oa dae o kopantsoeng le 4-AA;leha ho le joalo, 4-AA Tharollo e tsitsitse ho feta tharollo ea MBTH.Substrate e entsoe ka enzymatic oxidized ka oxidase ea eona ho hlahisa hydrogen peroxide.Motsoako oa peroxide ena ea hydrogen o lumellana le mahloriso a substrate.Ka hona, palo ea substrate e ka khethoa ke nts'etsopele ea 'mala oa karabelo ea oxidative coupling.Glucose, joala, acyl-CoA, le k'holeseterole li ka sebelisoa ho bona li-substrates tseo li kopantsoe le li-reagents tsa "Trinder" le 4-AA.Ho na le li-reagents tse ncha tse 10 tsa Trinder.Har'a li-reagents tse ncha tsa Trinder, TOOS ke eona e sebelisoang haholo.Leha ho le joalo, bakeng sa substrate e itseng, ho hlokahala liteko tsa mefuta e fapaneng ea li-reagents tsa Trinder ho nts'etsapele sistimi e nepahetseng ea ho lemoha.
