Lihlahisoa

Sigma receptor 1 (σR1) ke protheine ea transmembrane e seng ea opioid e ka sebetsang e le molek'hule molek'hule ho endoplasmic reticulum-mitochondrial membrane.Ligands bakeng sa σR1, tse kang (+)-pentazocine [(+)-PTZ], li fana ka tšireletso ea retinal neuroprotection in vivo le in vitro.Haufinyane tjena re ile ra sekaseka retinal phenotype ea litoeba tse haellang σR1 (σR1 KO) mme ra hlokomela morphology e tloaelehileng ea retinal le ts'ebetso ho litoeba tse nyane (libeke tse 5-30) empa ra fokotsa likarabo tse mpe tsa scotopic threshold (nSTRs), tahlehelo ea retinal ganglion cell (RGC) le tšitiso. ea optic nerve axon e tsamaellanang le ho se sebetse hantle ha retina ka selemo se le seng.Lintlha tsena li ile tsa etsa hore re hlahlobe khopolo ea hore σR1 e ka 'na ea e-ba ea bohlokoa ho thibela khatello ea kelello e sa foleng ea retina;Ho fumana hore na σR1 ea hlokahala bakeng sa (+) -PTZ litlamorao tsa neuroprotective, li-RGC tsa mantlha tse arohaneng le mofuta o hlaha (WT) le litoeba tsa σR1 KO li ile tsa pepesetsoa xanthine-xanthine oxidase (10 µM: 2 mU/ml) ho baka khatello ea oxidative boteng kapa ho ba sieo ha (+) -PTZ.Lefu la sele le ile la hlahlojoa ka tlhahlobo ea terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL).Ho lekola litlamorao tsa khatello ea maikutlo e sa foleng ts'ebetsong ea RGC, lefu la tsoekere le ile la kenngoa libeke tse 3 tsa C57BL/6 (WT) le σR1 KO litoeba, ho sebelisa streptozotocin ho hlahisa lihlopha tse nne: WT nondiabetic (WT non-DB), WT diabetic (WT-DB) ), σR1 KO non-DB, le σR1 KO-DB.Ka mor'a libeke tsa 12 tsa lefu la tsoekere, ha litoeba li ne li le libeke tse 15, khatello ea intraocular (IOP) e ile ea tlalehoa, tlhahlobo ea electrophysiologic e ile ea etsoa (ho akarelletsa le ho lemoha nSTRs), 'me palo ea RGC e ne e baloa likarolong tsa retinal histological. (+) -PTZ ha e khone ho thibela lefu le bakoang ke khatello ea kelello ea oxidative ea li-RGC tse kotuloang ho tloha ho litoeba tsa σR1 KO empa ea fana ka tšireletso e matla khahlanong le lefu la RGC e kotuloang litoeba tsa WT.Lithutong tsa khatello ea maikutlo e sa foleng e bakoang ke lefu la tsoekere, IOP e lekantsoeng ka lihlopha tse 'nè tsa litoeba e ne e le ka har'a mefuta e tloaelehileng;leha ho le joalo, ho bile le keketseho e kholo ea IOP ea σR1 KO-DB litoeba (16 ± 0.5 mmH g) ha e bapisoa le lihlopha tse ling tse lekiloeng (σR1 KO non-DB, WT non-DB, WT-DB: ~12 ± 0.6 mmHg ).Mabapi le tlhahlobo ea electrophysiologic, li-nSTRs tsa σR1 KO litoeba tse sa DB li ne li tšoana le litoeba tsa WT tseo e seng tsa DB libeke tsa 15;leha ho le joalo, li ne li le tlase haholo ho σR1 KO-DB litoeba (5 ± 1 µV) ha li bapisoa le lihlopha tse ling, ho kenyeletsoa, ​​haholo-holo, σR1 KO-nonDB (12±2 µV).Joalokaha ho lebeletsoe, palo ea RGCs ho σR1 KO litoeba tseo e seng tsa DB li ne li tšoana le litoeba tsa WT tseo e seng tsa DB libeke tsa 15, empa tlas'a khatello ea kelello e sa foleng ea lefu la tsoekere ho ne ho e-na le RGC e fokolang ho retinas ea σR1 KO-DB litoeba. Ena ke tlaleho ea pele. ho bontša ka mokhoa o sa tsitsang hore liphello tsa neuroprotective tsa (+) -PTZ li hloka σR1.σR1 KO litoeba li bonts'a sebopeho se tloaelehileng sa retinal le ts'ebetso ho sa le banyenyane;leha ho le joalo, ha u le tlas'a khatello ea maikutlo e sa foleng ea lefu la tsoekere, ho na le keketseho ea bofokoli ba ts'ebetso ea retinal ho σR1 KO litoeba hoo ho se sebetse hantle ha lisele tsa ganglion ho bonoa lilemong tsa pejana ho feta litoeba tsa nondiabetic σR1 KO.Lintlha li tšehetsa khopolo ea hore σR1 e phetha karolo ea bohlokoa ho modu lating khatello ea kelello 'me e ka' na ea e-ba sepheo sa bohlokoa bakeng sa lefu la retinal.