TMB Cas: 54827-17-7 99% E tšoeu, e tšoeu ho ea ho e putsoa kapa e mosehla
Nomoro ea lethathamo | XD90163 |
Lebitso la Sehlahiswa | TMB |
CAS | 54827-17-7 |
Foromo ea limolek'hule | C16H20N2 |
Boima ba Molek'hule | 240.34 |
Lintlha tsa polokelo | 2 ho isa ho 8 °C |
Khoutu ea Lekhetho e Kopantsoeng | 29215990 |
Tlhaloso ea Sehlahisoa
Ponahalo | Bosoeu, bosoeu ho isa ho bohlooho bo boputsoa kapa phofo e mosehla |
Assay | 99% |
Tahlehelo ka ho Omisa | <2.0% |
Bakeng sa tšebeliso ea lipatlisiso feela, eseng molemong oa batho | lipatlisiso li sebelisoa feela, eseng molemong oa batho |
Thepa: 3,3',5,5'-Tetramethylbenzidine ke phofo e tšoeu ea crystalline, ha e na monko, ha e na tatso, ha e qhibilihe ka metsing, e qhibiliha habonolo ka acetone, ether, dimethyl sulfoxide, dimethylformamide, joalo-joalo.
Boitokisetso: 3,3,5,5-Tetramethylbenzidine ke reagent ea bohlokoa ea chromogen.Ho sebelisa 2,6-dimethylaniline e le lisebelisoa tse tala, ka ho kenya ts'ebetsong, ho kopanya oxidative le ho hloekisa, ho fumanoa 3,3,5,5-tetramethylbenzidine e hloekileng, 'me kakaretso ea lihlahisoa e fihla ho 65%.
Ts'ebetso ea baeloji: TMB (BMblue) ke karoloana ea chromogenic bakeng sa immunohistochemistry le ELISA.
Lisebelisoa: Sehlahisoa sena ke sebaka se seng sa carcinogenic bakeng sa benzidine 1 (Ames test negative), se loketse joalo ka peroxidase substrate bakeng sa tlhahlobo ea immunosorbent e amanang le enzyme.Substrate e fana ka sehlahisoa sa ho qetela se soluble bluish se ka baloang spectrophotometrically ho 370 kapa 620-650 nm.Maikutlo a TMB a ka emisoa ka 2MH2SO4 (e fetoha mosehla) 'me a baloe ho 50 nm ho 4Chemicalbook.Reagent e hlokolosi le e khethehileng bakeng sa ho bona mali, ho metha hemoglobin, le ho metha peroxidase.
Lisebelisoa: Sesebelisoa se secha se bolokehileng sa chromogen;TMB butle-butle e nkile sebaka sa benzidine e matla ea carcinogen le lintho tse ling tse tsoang ho carcinogen benzidine, 'me e sebelisoa litekong tsa bongaka, tlhahlobo ea forensic, tlhahlobo ea botlokotsebe le tlhahlobo ea tikoloho le mafapha a mang;haholo-holo litekong tsa kliniki tsa biochemical, TMB, e le substrate e ncha ea peroxidase, e sebelisitsoe haholo ho enzyme immunoassay (EIA) le enzyme-linked immunosorbent assay (ELISA);e sebelisoa haholo-holo mererong e latelang: ho lemoha menoana ea mali ea boloi;Ho lemoha ka potlako joala ka mathe;ho lokisoa ha lihlopha tsa tlhahlobo ea moroto;ho fumanoa ha kokoana-hloko ea hepatitis;teko ea ho lemoha moimana;boikemisetso bo potlakileng ba tsoekere, hemoglobin, albin maling le moroto;teko ea mali ea boloi ea mantle;boikemisetso ba boleng ba granulocyte maling, li-steroids, Ho fumanoa ha lihomone tsa thobalano;boikemisetso ba mosebetsi oa enzymatic;ho fumana li-antigen, li-antibodies le liphatsa tsa lefutso