Lihlahisoa

1.Optically active D-2-hydroxybutanoate ke moaho oa bohlokoa oa mahareng bakeng sa meriana le biodegradable poly(2-hydroxybutanoate).Qeto ea Kinetic ea racemic 2-hydroxybutanoate e ka ba mokhoa o mong o motala le o lakatsehang bakeng sa tlhahiso ea D-2-hydroxybutanoate.Mosebetsing ona, D-2-hydroxybutanoate sebakeng se phahameng (0.197 M) le tekanyo e phahameng ea enantiomeric (99.1%) e hlahisitsoe ke L-lactate dehydrogenase e ikemetseng (L-iLDH) e nang le biocatalyst e ikemetseng ea NAD.2-Oxobutanoate, e 'ngoe ea bohlokoa e bohareng, e ile ea hlahisoa ka bongata bo bongata (0.193 M).Ho sebelisa mokhoa o bonolo oa phapanyetsano ea ion le macroporous anion exchange resin D301, D-2-hydroxybutanoate e ile ea arohanngoa le tsamaiso ea biotransformation e nang le pholiso e phahameng ea 84.7%.

2.Asymmetric synthesis ea amino acid e seng ea tlhaho e ile ea bontšoa ke omega-transaminase ho tloha Vibrio fluvialis JS17.L-2-Aminobutyric acid e entsoe ho tloha 2-oxobutyric acid le benzylamine e nang le senantiomeric e feteletseng ho feta 99%.Boitšoaro bo ile ba bontša thibelo e matla ea sehlahisoa ka benzaldehyde, e ileng ea hlōloa ka ho sebelisa mokhoa oa biphasic reaction system ho tlosa sehlahisoa sa inhibitory ho tloha karolong ea metsi.Ka mokhoa o tloaelehileng oa biphasic reaction (50 mM 2-oxobutyric acid, 70 mM benzylamine le 2.64 U / ml enzyme e hloekisitsoeng) e sebelisa hexane e le motsoako, phetoho ea 2-oxobutyric acid e fihlile 96% ka 5 h athe phetoho ea 39% feela e ile ea fumanoa ntle le tlhahiso ea lihlahisoa.