Streptomycin sulfate CAS: 3810-74-0 Phofo e tšoeu
| Nomoro ea lethathamo | XD90361 |
| Lebitso la Sehlahiswa | Streptomycin sulfate |
| CAS | 3810-74-0 |
| Foromo ea limolek'hule | (C21H39N7O12)2·3H2SO4 |
| Boima ba Molek'hule | 1457.39 |
| Lintlha tsa polokelo | 2 ho isa ho 8 °C |
| Khoutu ea Lekhetho e Kopantsoeng | 29412080 |
Tlhaloso ea Sehlahisoa
| Kereiti | USP36/EP8.0 |
| pH | 4.5-7.0 |
| Tahlehelo ka ho Omisa | ≤7.0% |
| Tlhahlobo | ≥720IU/MG |
| Sulfate | 18.0 ~ 21.5% |
| Ponahalo | Phofo e tšoeu kapa e batlang e le tšoeu, e qhibiliha haholo ka metsing, e batlang e sa qhibilihe ka har'a ethanol. |
| Methanol | ≥0.3% |
| Streptomycin B | ≤3.0% |
| Teko ea Colorimetric | ≥90.0% |
| Asiti ea sulfuric | ≤1.0% |
| Tekanyo e phahameng ea sodium sulphite | ≤0.4% |
Le hoja etiology ea intervertebral disc degeneration e sa utloisisoe hantle, mokhoa o mong oa ho thibela ts'ebetso ena e ka 'na ea e-ba ho thibela apoptosis.Phuputsong ea morao-rao, liphello tsa anti-apoptotic tsa carboxymethylated chitosan (CMCS) liseleng tsa nucleus pulposus (NP) li ile tsa hlahlojoa ka sepheo sa ho ntlafatsa bophelo ba lisele tsa disc.Lisele tsa Rat NP li ne li khethiloe 'me tsa lengoa ka in vitro,' me hydrogen peroxide (H2O2) e ile ea sebelisoa ho haha mohlala oa NP cell apoptosis.Ho sebetsa ha lisele ho ile ha lekoa ka tlhahlobo ea ho bala lisele-8.Karo-karolelano ea lisele tsa apoptotic e ile ea hlahlojoa ke annexin V-fluorescein isothiocyanate (FITC) le propidium iodide (PI) tlhahlobo ea mabala a mabeli, 'me morphology e ile ea hlokomeloa ke Hoechst 33342 staining.Bokhoni ba lera la mitochondrial la lisele tsa NP bo ile ba hlahlojoa ke rhodamine 123 fluorescence staining.Reverse transcription (RT)‑quantitative polymerase chain reaction (qPCR) e entsoe ho lekanya maemo a mRNA a inducible nitric oxide synthase (iNOS), caspase-3, B-cell lymphoma (Bcl)‑2, mofuta oa II collagen le aggrecan.Tlhahlobo ea blot ea Bophirimela e ile ea etsoa ho fumana maemo a protheine ea iNOS le Bcl-2.Liphetho tsa annexin V-FITC/PI le Hoechst 33342 li bonts'itse hore CMCS e khonne ho thibela lisele tsa NP ho tloha apoptosis ka mokhoa o itšetlehileng ka lethal dose.Rhodamine 123 staining e hlakisitse hore CMCS e fokolitse ho senyeha ha lera la mitochondrial liseleng tsa NP tse phekoloang ke H2O2.Ho fokotseha ha caspase‑3 le ts'ebetso e ntseng e eketseha ea Bcl-2 li fumanoe liseleng tsa NP tse tšoaroang ke CMCS ke RT-qPCR le tlhahlobo ea blot ea bophirima.CMCS e boetse e khothalelitse ho ata le ho hlahisoa ha mofuta oa II collagen le aggrecan liseleng tsa NP tse tšoaroang ke H2O2.CMCS e bontšitsoe hore e atlehile ho thibela lefu la apoptotic cell in vitro, ho bontša melemo e ka bang teng ea mokhoa ona oa phekolo ho laola ho senyeha ha disc.
