Lihlahisoa

Acridine ester (NSP-SA-NHS) Cas199293-83-9 le metsoako ea eona e amanang le eona ke li-label tsa chemiluminescent tse molemo haholo tseo botsitso, mosebetsi le kutloisiso li fetang li-radioisotopes tse ling.Acridine esters e ka itšoara ka liprotheine tse nang le lihlopha tsa mantlha tsa amino.Tlas'a maemo a motheo, NHS e nkeloa sebaka e le sehlopha se tlohang, 'me protheine e etsa tlamo e tsitsitseng ea amide le ester ea acridine.Ka mor'a hore karabelo e phethoe, letsoai le feteletseng la acridinium le ile la tlosoa ka mohala oa desalting.

Liprotheine tse ngotsoeng ka Acridine ha li hloke hore catalysis ea enzymatic e hlahise leseli ka pel'a alkaline hydrogen peroxide.Molao-motheo o khethehileng oa ho ntša khanya ke hore ka tharollo ea alkaline ea hydrogen peroxide, ester ea acridine e hlaseloa ke li-ion tsa hydrogen peroxide ho hlahisa dioxyethane e sa tsitsang e nang le tsitsipano, e ntseng e senyeha ka ho eketsehileng ho CO2 le acridone e thabisang ea elektronike.Ha acridone e khutlela sebakeng sa fatše, e hlahisa li-photon tse nang le maqhubu a mangata a ho monya a 430 nm.Ts'ebetso ena ea luminescence e khuts'oane haholo (ts'ebetso eohle e nka metsotsoana e ka tlase ho 2), 'me leano la ho qalisa le tlameha ho eketsa photometer ea ka hare le mochine oa photon;ho phaella moo, sehlahisoa sena se ka boela sa sebelisa sebali sa microplate se nang le lisebelisoa tse ngata tse nang le mochine oa autosampler bakeng sa pokello ea lintlha tsa luminescence.Liprotheine, li-peptide, li-antibodies, le li-nucleic acid kaofela li ka ngoloa ka sehlahisoa sena.Acridine esters e ntša khanya ka potlako tlas'a ho tsosoa ha alkaline hydrogen peroxide, kahoo metsoako e ngotsoeng e ka fumanoa ka ho bokella photon.

Sehlahisoa sena se sebelisoa haholo-holo bakeng sa: chemiluminescence le immunoassay, tlhahlobo ea li-receptor, nucleic acid le ho lemoha peptide le lipatlisiso tse ling.