Lihlahisoa

Ho ile ha etsoa tlhahlobo e bonolo ea sebaka, e lumellang hore ho be le palo ea microalbuminuria.Tlhahlobo e ile ea etsoa ho latela melaoana ea ISO 15189. Moroto o ile oa bonoa makhapetla a selulose acetate ’me o silafalitsoe ho sebelisoa lidae tse tlamang liprotheine tse fapaneng (nigrosin, Coomassie Blue R-250, amido black).Matla a 'mala a matheba a matheba a ile a lekanngoa ho sebelisoa seteishene sa Kodak Image 450. Analytical sensitivity of the Coomassie Blue based method (18 mg/L) e ne e le betere ho feta ea nigrosin (50 mg/ L) kapa amido black (100 mg/L) ) mekhoa e thehiloeng.Ka har'a coefficient ea phapang (CV) le lipakeng tsa CV ea tlhahlobo e putsoa ea Coomassie e ne e le, ka ho latellana, 8.4% le 9.7% (50 mg/L), le 3% le 4.5% (400 mg/L).Bakeng sa nigrosin, lintlha tsena e ne e le 8.4 le 9.4 (50 mg / L), le 3.4 le 6.4% (400 mg / L).Coomassie Blue e bontšitse khetho e tlamang e ikhethileng mabapi le albumin.Mokhoa o fumanoe o le mola pakeng tsa 20 le 600 mg/L.Kamano e ntle (r2 = 0.89) e ile ea fumanoa pakeng tsa Coomassie Blue e thehiloeng le litekanyo tsa immunonephelometric.Immuno-unreactive albumin (e lokisitsoeng ke kalafo ea protease) e ka bonoa ka tlhahlobo ea letheba, e fanang ka monyetla oa mokhoa o bapisoang le liteko tsa immunochemical.Ammonium sulphate precipitation e ka eketsa ts'ebetso ea tlhahlobo ka ho felisa litlamorao tsa liketane tsa leseli tse sa lefelloeng. Mokhoa o hlalositsoeng o bonolo haholo ebile o theko e tlase haholo, e leng se etsang hore e tšoanelehe bakeng sa mananeo a tlhahlobo, haholo-holo linaheng tsa boraro.