Lihlahisoa

Ho hlahloba ka mokhoa o hlophisehileng litšobotsi tse silafatsang le polokeho ea lidae tse ncha tse ka bang teng bakeng sa opereishene ea intraocular. Lidae tse tšeletseng li kenyellelitsoe lipatlisisong: botala bo botala bo bobebe SF (LGSF) bo mosehla, E68, bromophenol blue (BPB), Chicago blue (CB), rhodamine 6G, rhodulinblau -ea motheo 3 (RDB-B3).Lidae tsohle li ile tsa qhibiliha 'me tsa hlapolloa ka motsoako oa saline saline e leka-lekaneng.Thepa ea ho monya khanya ea dae e 'ngoe le e' ngoe e lekantsoe ka bongata ba 0.05% pakeng tsa 200 le 1000 nm.Litšobotsi tse silafatsang li ile tsa hlahlojoa ka ho silafatsa lithane tsa lense tsa capsule le li-membrane tsa epiretinal (ERMs), li tlositsoe ka mokhoa o ts'oanang, ka likhakanyo tsa dae tsa 1.0%, 0.5%, 0.2%, le 0.05%.Mahlo a porcine a enucleated (nako ea postmortem, lihora tsa 9) le tsona li ne li silafalitsoe.Chefo e amanang le dae e ile ea hlahlojoa ka tlhahlobo ea colorimetric (MTT) e lekanyang ho thibela ho ata ha lisele tsa retinal pigment epithelium (RPE) (ARPE-19 le lisele tse ka sehloohong tsa RPE tsa motho, litemana tsa 3-6).Ho sebetsa ha lisele ho boetse ho lekanyelitsoe ho ipapisitsoe le tlhahlobo ea matla a lisele tsa fluorescence ea mebala e 'meli.Lidae li ile tsa batlisisoa ka likhakanyo tsa 0.2% le 0.02%. Lidae tsohle tse hlahlobiloeng phuputsong ena li ile tsa silafatsa li-capsules tsa lense tsa motho, tsa tlosoa ka har'a ts'ebetso;Li-ERM, tse ebotsoeng nakong ea opereishene ea macular pucker;le enucleated porcine mahlo, ho itšetlehile ka mahloriso sebelisoa.Bophahamo ba nako e telele ea ho monya lidae bo ne bo le ka har'a 527 ho isa ho 655 nm maemong a 0.05%.Rhodamine G6 le RDB-B3 li bontšitse liphello tse mpe ka ho ata ha lisele tsa ARPE-19 ka har'a mahloriso a 0.2% 'me li ne li sa kenyelletsoa lipatlisisong tse eketsehileng liseleng tsa mantlha tsa RPE.Lidae tse 'nè tse setseng ha lia ka tsa bontša phello e chefo ho ARPE-19 le ho ata ha lisele tsa mantlha tsa RPE maemong a 0.2% le 0.02%.Tšebeliso ea lisele e ile ea angoa ke LGSF yellowish (0.2%) le CB (0.2% le 0.02%).Lidae tse peli (E68 le BPB) ha lia ka tsa bontša chefo e amehang ka har'a vitro. Tekolo e hlophisitsoeng ea lidae bakeng sa tšebeliso ea intraocular e bonahala e tlama.Thutong ena ho ile ha khetholloa lidae tse 'nè tse nang le litšobotsi tse sebetsang hantle tsa litšila, 'me tse peli tsa lidae tsena li se na chefo e bonoang liseleng tsa RPE in vitro.