L-Leucine Cas: 61-90-5 99% phofo e tšoeu
| Nomoro ea lethathamo | XD90304 |
| Lebitso la Sehlahiswa | L-Leucine |
| CAS | 61-90-5 |
| Foromo ea limolek'hule | C6H13NO2 |
| Boima ba Molek'hule | 131.17292 |
| Lintlha tsa polokelo | Ambient |
| Khoutu ea Lekhetho e Kopantsoeng | 29224985 |
Tlhaloso ea Sehlahisoa
Joalo ka livaerase tse ling tsa RNA tse nang le li-positive, li-tombusvirus li kopitsoa ke "membrane-bound viral replicase complex" (VRC).VRC e na le RNA polymerase e itšetlehileng ka kokoana-hloko ea p92 (RdRp), kokoana-hloko ea p33 RNA chaperone, le liprotheine tse 'maloa tse kopanetsoeng.E le hore e be RdRp e sebetsang ka mor'a phetolelo ea eona, protheine ea p92 ea ho pheta-pheta e lokela ho kenngoa ho VRC, e lateloe ke ts'ebetso ea eona.Re kile ra bonts'a tekong e thehiloeng ho sele e sa hlokeng tomoso ea tomoso hore ts'ebetso ea Tomato bushy stunt virus (TBSV) RdRp e hloka soluble host factor(s).Sengoliloeng sena, re supa cellular shock shock protein 70 (Hsp70) e le co-opted host factor e hlokahalang bakeng sa ho kenya tšebetsong N-terminally truncated recombinant TBSV RdRp.Ntle le moo, thibelo e thehiloeng ho molek'hule e nyane ea ts'ebetso ea Hsp70 e thibela motsoako oa RNA ke tombusvirus RdRp in vitro.Ho feta moo, re bontša hore li-phospholipids tse sa nke lehlakore, e leng, phosphatidylethanolamine (PE) le phosphatidylcholine (PC), li ntlafatsa ts'ebetso ea RdRp in vitro.Ka lehlakoreng le leng, phosphatidylglycerol (PG) e bonts'a phello e matla le e matla ea inhibitory ts'ebetsong ea in vitro RdRp.Re boetse re bonts'a hore PE le PC li hlohlelletsa likamano tsa RdRp-viral plus-strand RNA [(+)RNA], ha PG e thibela ho tlama RNA ho RNA ho RdRp.Ho ipapisitsoe le likarolo tse fapaneng tsa phospholipids ho tombusvirus RdRp activation, re sisinya hore sebopeho sa lipid sa li-membrane tsa subcellular tse lebisitsoeng li ka sebelisoa ke tombusviruses ho laola kopano e ncha ea VRC nakong ea ts'oaetso. (RdRp), e ikarabellang bakeng sa ho kopanya likokoana-hloko tsa RNA liseleng tse tšoaelitsoeng tsa livaerase tse 'maloa tsa mefuta e metle ea RNA, qalong ha e sebetse.Leano lena le ka 'na la qoba motsoako oa likokoana-hloko oa RNA ho cytosol e neng e tla lebisa ka potlako ho kenngoa ha RNA-triggered cellular responses antiviral.Nakong ea kopano ea "membrane-bound replicase complex", "viral RdRp" e sebetsa ka mokhoa o sa utloisisoeng hantle o etsang hore RdRp e khone ho kopanya RNA.Ka ho sebelisa TBSV RdRp, re bonts'a hore mohalaleli oa cellular oa Hsp70 le li-phospholipids tse sa nke lehlakore li thusa ho kenya tšebetsong RdRp in vitro.Ka lehlakoreng le leng, phosphatidylglycerol (PG) e na le phello e matla ea inhibitory ho in vitro RdRp activation le RdRp-viral RNA interaction, e fana ka maikutlo a hore microdomain ea membranous e potolohileng RdRp e ama haholo bokhoni ba eona ba ho kopanya RNA.Kahoo, ts'ebetso ea vaerase ea RdRp mohlomong e ipapisitse le likarolo tse ngata tse amohelang lisele tse nang le tšoaetso.
4-Nitrophenyl-N-acetyl-β- D-glucosaminide ke substrate e molemo bakeng sa tlhahlobo ea colorimetric e potlakileng ea mosebetsi oa N-acetyl-b-glucosaminidase morong oa motho.Chromogenic β-Glucosaminidase substrate e fana ka tharollo e mosehla ha e petsoha, e sebelisoang haholo-holo tomoso le hlobo.
