Lihlahisoa

Adenosine ke endogenous regulator ea vascular tone.Mosebetsi ona oa adenosine o felisoa ke ho kenngoa ha oona le metabolism ka lisele tsa microvascular endothelial (MVEC).Sepalangoang se ka sehloohong se amehang ke ENT1 (equilibrative nucleoside transporter subtype 1).MVEC e boetse e hlalosa nucleobase transporter (ENBT1) e amehang ho phalla ha lisele tsa adenosine metabolites tse kang hypoxanthine.Liphetoho ho e 'ngoe ea litsamaiso tsena tsa lipalangoang li ka ama bioactivity ea adenosine le metabolism ea eona, ho kenyelletsa le ho theha li-radicals tsa mahala tsa oksijene.MVEC e arohaneng le mesifa ea skeletal ea ENT1 (+/+) le ENT1 (-/-) litoeba li ne li le tlas'a khatello ea oxidative e bakoang ke ketsiso ea ischemia / reperfusion kapa menadione.Mesebetsi ea ENT1 le ENBT1 e hlahlobiloe ho ipapisitsoe le zero-trans influx kinetics ea li-substrates tse ngotsoeng ka radio.Ho bile le phokotseho ea sekhahla sa ENBT1-mediated hypoxanthine uptake ke ENT1(+/+) MVEC e phekoloang ka menadione kapa ka mor'a ho ba le maemo a etsisang isc hemia/reperfusion.Maemong ana ka bobeli, superoxide dismutase mimetic MnTMPyP e fokolitse tahlehelo ea ts'ebetso ea ENBT1, e kenyelletsang li-radicals tsa superoxide karabong.Ka lehlakoreng le leng, MVEC e arohaneng le ENT1(-/-) litoeba ha ea ka ea bontša phokotso ea ts'ebetso ea ENBT1 nakong ea kalafo ka menadione kapa ketsiso ea ischemia/reperfusion, empa e bile le boemo bo holimo haholo ba ts'ebetso ea catalase e amanang le ENT1(+/+) MVEC.Lintlha tsena li fana ka maikutlo a hore mosebetsi oa ENBT1 o fokotsehile ho MVEC ka lebaka la ho eketseha ha superoxide radical e amanang le kotsi ea ischemia / reperfusion.MVEC e arohaneng le ENT1(-/-) litoeba ha e bontše phokotso ena ho ENBT1, mohlomong ka lebaka la ts'ebetso e eketsehileng ea catalase.