GSH Cas: 70-18-8 98% phofo e tšoeu ea kristale
| Nomoro ea lethathamo | XD90227 |
| Lebitso la Sehlahiswa | GSH |
| CAS | 70-18-8 |
| Foromo ea limolek'hule | Setšoantšo sa C10H17N3O6S |
| Boima ba Molek'hule | 307.323 |
| Lintlha tsa polokelo | 2 ho isa ho 8 °C |
| Khoutu ea Lekhetho e Kopantsoeng | 29309098 |
Tlhaloso ea Sehlahisoa
| Tlhaloso ea Sehlahisoa | |
| Melting Point | 185°C |
| Kereiti | Sehlopha sa Pharma |
| Litšepe tse boima | 10ppm boholo |
| Arsenic | 2ppm boholo |
| Boitsebiso | IR : E tšoana le Reference Spectrum |
| Tahlehelo ka ho Omisa | 0.5% boholo |
| Mocheso oa polokelo | +20 ° C |
| Boima ba Molek'hule | 612.63 |
| Tlhahlobo | 98.0% mets |
| Ho potoloha ha Optical | -15.5°- -17.5 deg |
| Masalla a ho Ignition | 0.1% boholo |
| Ho Hlakola ha Tharollo | E hlakileng ebile e se na 'mala |
| Ponahalo | Phofo e tšoeu ea kristale |
| Ntho e Amanang | Kakaretso: 2.0% max, GSSG:1.5% max |
Protheine ea ORF45 ea Kaposi's sarcoma-associated herpesvirus (KSHV) ke protheine ea tegument ea pele-pele ea gammaherpesvirus.Lithuto tsa rona tse fetileng li senotse likarolo tsa eona tsa bohlokoa maemong a pele le a morao-rao a ts'oaetso ea KSHV.Thutong ena, re hlahlobile interactome ea ORF45 re sebelisa sehlopha sa li-antibodies tsa monoclonal.Ho phaella ho liprotheine tse tsejoang pele tsa extracellular regulated kinase (ERK) le p90 ribosomal S6 kinase (RSK), re fumane liprotheine tse ling tse 'maloa tse kopantsoeng, ho akarelletsa le tse hlaheletseng tsa ~ 38 kDa le ~130 kDa.Mass spectrometry e senoletse hore protheine ea 38-kDa ke kokoana-hloko ORF33 le protheine ea 130-kDa ke cellular USP7 (ubiquitin-specific protease 7).Re ile ra etsa 'mapa oa sebaka se tlamang sa ORF33 ho carboxyl-terminal 19 amino acid (aa) e bolokiloeng haholo ea ORF45 le sebaka se tlamang sa USP7 ho sepheo se tlalehiloeng sa tumellano sebakeng se bohareng sa ORF45.Re sebelisa immunofluorescence staining, re bone colocalization ea ORF45 le ORF33 kapa USP7 ka bobeli tlas'a li-ion tse tšoaetsanoang le lisele tse nang le tšoaetso ea KSHV.Ho feta moo, re hlokometse ho fallisoa ho itšetlehileng ka ORF45 ea karolo ea ORF33 / USP7 ho tloha nucleus ho ea cytoplasm.Re fumane hore ORF45 e bakile ho eketseha ha protheine ea ORF33 e ileng ea felisoa haeba sebaka sa ORF33- kapa sa USP7-binding se ORF45 se hlakotsoe.Ho feta moo, ho hlakoloa ha carboxyl terminus e bolokiloeng ea ORF45 ho genome ea KSHV ho fokolitse haholo boemo ba protheine ea ORF33 liseleng tse nang le tšoaetso ea KSHV le ho felisa tlhahiso ea li-virion tsa bana.Ka kopanelo, liphetho tsa rona ha li senole feela likarolo tse ncha tsa ORF45 interactome, empa hape li bontša hore tšebelisano har'a liprotheine tsena ke tsa bohlokoa bakeng sa ho pheta-pheta ha KSHV.KSHV ORF45 ke protheine e sebetsang ka bongata e hlokahalang bakeng sa ho ikatisa ha KSHV lytic, empa mekhoa e nepahetseng eo ORF45 e etsang mesebetsi ea eona ea bohlokoa ha e hlake.Liphuputso tsa rona tse fetileng li senoletse hore ORF45 pr otein eohle ka liseleng e teng ka har'a li-complexes tse boima ba limolek'hule.Ka hona re ile ra batla ho tsebahatsa "interome" ea ORF45 ho fana ka leseli mabapi le mesebetsi ea eona nakong ea phetisetso ea lytic.Re sebelisa sehlopha sa li-antibodies tsa monoclonal, re ile ra hlahloba ORF45 interactome liseleng tse nang le tšoaetso ea KSHV.Re khethile balekane ba babeli ba bacha ba tlamang ba ORF45: protheine ea vaerase ORF33 le cellular ubiquitin-specific protease 7 (USP7).Re tsoela pele ho bontša hore tšebelisano pakeng tsa ORF45 le ORF33 ke ea bohlokoa bakeng sa tlhahiso e sebetsang ea likokoana-hloko tsa KSHV, ho fana ka maikutlo a hore tšitiso e lebisitsoeng le tšebelisano ena e ka 'na ea emela leano le lecha la ho thibela ho ikatisa ha KSHV lytic.
