Deoxyribonucleic acid, peo ea tlhapi Cas:100403-24-5
| Nomoro ea lethathamo | XD90579 |
| Lebitso la Sehlahiswa | Deoxyribonucleic acid, peō ea tlhapi |
| CAS | 100403-24-5 |
| Foromo ea limolek'hule | - |
| Boima ba Molek'hule | - |
| Lintlha tsa polokelo | 2 ho isa ho 8 °C |
| Khoutu ea Lekhetho e Kopantsoeng | 29349990 |
Tlhaloso ea Sehlahisoa
| Ponahalo | Phofo e tšoeu |
| Tlhahlobo | 99% |
Litsela tsa limolek'hule tsa pathogenic ho mafu a sebete a ho thatafala a kang kokoana-hloko ea hepatitis C (HCV), lefu la sebete la autoimmune (AIH) le primary biliary cirrhosis (PBC) ha li tsejoe hantle.Liphatsa tsa lefutso tse fapaneng hangata li bohlokoa ho pathogenesis ea mafu.Suppression subtractive hybridization (SSH) ke mokhoa o pharalletseng oa liphatsa tsa lefutso o ntlafatsang lingoloa tsa mRNA tse hlahisitsoeng ka mokhoa o fapaneng.Re ne re ikemiselitse ho etsa litebello tse ncha tsa polelo ea phapano ea lefutso ho cirrhosis re sebelisa SSH e kopantsoeng le quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR).Li-transcriptomes tsa sebete ho HCV cirrhosis, AIH cirrhosis, PBC, le lisele tsa sebete tse sa sebetseng li ile tsa hlahlojoa ke SSH.Li-cDNA tse tlatsanang tsa DNA (cDNA) li ile tsa hlahlojoa bocha bakeng sa polelo e fapaneng ka ho kopanngoa ha matheba a matheba 'me tsa hlophisoa.Polelo ea liphatsa tsa lefutso e khethiloeng e ile ea lekanyetsoa ke RT-PCR ea nako ea sebele.Ka mor'a SSH, li-clone tse 694 li ile tsa hlahlojoa bocha bakeng sa polelo e fapaneng ea liphatsa tsa lefutso, tseo tse 145 tsa tsona li ileng tsa hlophisoa 'me tsa fumanoa li tsoa liphatseng tse fapaneng tse 89.Li-clone tse supileng li ne li tšoana feela ka tag ea tag (EST) ea tatelano ea liphatsa tsa lefutso tse se nang tšebetso e tsejoang.Polelo e laoloang ka holimo ea liphatsa tsa lefutso tse 'nè e tiisitsoe ke RT-PCR ea nako ea sebele: transmembrane 4 setho sa superfamily 3 (tetraspanin CO-029) ka mefuta eohle ea cirrhosis, hedgehog interacting protein (HIP) ho AIH cirrhosis le chitinase 3-like-1 (HC gp-39 kapa ykl-40) le arginine-glutamic acid pheta (RERE) ho HCV cirrhosis.RERE liphatsa tsa lefutso tsa polymorphisms le mefuta e fapaneng ea li-splice li ile tsa bonoa liseleng tsohle tse hlahlobiloeng.Tetraspanin CO-029 up-regulation e ne e le sebakeng sa lisele tsa bile ductular.Qetellong, litebello tse ncha tsa polelo e fapaneng ea lefutso ho cirrhosis ea motho li entsoe ho sebelisoa SSH e le sesebelisoa sa mantlha sa ho sibolla.Haholo-holo, lithuto tse ling tsa lefutso la RERE le lihlahisoa tsa eona ho lefu la sebete le amanang le HCV li nepahetse.
