Lihlahisoa

CTP:phosphocholine cytidylyltransferase α (CCTα) ke enzyme ea nyutlelie e susumetsang mohato oa ho fokotsa sekhahla ho CDP-choline pathway for phosphatidylcholine (PC) synthesis.Tšebeliso ea Lipid ea CCTα e fella ka hore e fetisetsoe ho enfelopo ea nyutlelie le katoloso ea marang-rang a lera la intranuclear le bitsoang nucleoplasmic reticulum (NR) ka mochine o kenyelletsang ho senyeha ha lera.Li-lamine tsa nyutlelie li boetse lia hlokahala bakeng sa botsitso le ho ata ha NR, empa hore na sebopeho sena se ikhethang, kapa lamina ea nyutlelie ka kakaretso, ea hlokahala bakeng sa motsoako oa PC ha e tsejoe.Ho hlahloba kamano ena, lamina ea nyutlelie e ile ea fokotsoa ke RNAi kapa ea sitisoa ke polelo ea Hutchinson-Gilford progeria syndrome (HGPS) mutant lamin A (progerin), 'me phello ea CCTα le choline metabolism e ile ea hlahlojoa.SiRNA-mediated ho khutsisa lamin A/C kapa lamin B1 liseleng tsa CHO ho fokotsa NR ha hoa ka ha e-ba le phello ho PC synthesis, ha ho kokota habeli ho sa thibele tsela ka ho khetheha.Ho netefatsa karolo ea hae e nyane ho PC synthesis, ke 10% feela ea choline/ethanolamine phosphotransferase e fetelletseng ea nakoana e fumanoeng ho NR.Liseleng tsa CHO, CCTα e ne e le nucleoplasmic le co-localized le GFP-progerin ka mameno a nyutlelie le litlhaselo;leha ho le joalo, HGPS fibroblasts e bontšitse kabo e sa tloaelehang ea CCTα ka har'a cytoplasm le enfelopo ea nyutlelie e neng e tsamaisana le phokotso ea 2 ho PC synthesis.Ho sa tsotellehe boemo ba eona bo fetotsoeng, liteko tsa choline-labeling li bontšitse hore mosebetsi oa CCT o ne o sa ameha, 'me ho thibela PC synthesis ho ile ha lateloa ke mosebetsi o fokolang oa hemicholinium-sensitive choline transporter.Re etsa qeto ea hore CCTα le lamine li sebelisana ka ho khetheha ho theha NR, empa sebopeho sa kakaretso sa enfelopo ea nyutlelie se na le tšusumetso e fokolang mosebetsing oa CCT le PC synthesis.