4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4 Off-White Powder 98%
| Nomoro ea lethathamo | XD90011 |
| Lebitso la Sehlahiswa | 4-Nitrophenyl-alpha-D-mannopyranoside |
| CAS | 10357-27-4 |
| Foromo ea limolek'hule | C12H15NO8 |
| Boima ba Molek'hule | 30301.25 |
| Lintlha tsa polokelo | -2ho -8°C |
| Khoutu ea Lekhetho e Kopantsoeng | 29400000 |
Tlhaloso ea Sehlahisoa
| Metsi | <5% Karl Fische |
| Solubility | 1% ho DMF e hlakile ebile ha e na 'mala |
| Bohloeki | Mahala 4-Nitrophenol <200ppm |
| HPLC | >98% |
| Ponahalo | Phofo e tšoeu |
Litlhaloso tsa mechine ho lelapa le itšetlehileng ka Ca2+ la alpha-mannosidase ka har'a symbiont ea mala a motho.
Libaktheria tsa Colonic, tse bontšitsoeng ke Bacteroides thetaiotaomicron, li phetha karolo ea bohlokoa ho boloka bophelo bo botle ba batho ka ho sebelisa malapa a maholo a glycoside hydrolases (GHs) ho sebelisa li-polysaccharides tsa lijo le ho amohela li-glycans e le limatlafatsi.Katoloso e joalo ea lelapa la GH e bonahatsoa ke lelapa la 23 la GH92 glycosidase le kentsoeng ke B. thetaiotaomicron genome.Mona re bonts'a hore tsena ke alpha-mannosidase tse sebetsang ka mokhoa o le mong oa ho falla ho sebelisa N-glycans e amohelang.Sebopeho sa mahlakore a mararo sa li-mannosidase tse peli tsa GH92 se hlalosa lelapa la liprotheine tse peli tsa li-domain moo setsi sa catalytic se fumanehang sebakeng sa marang-rang, se fana ka thuso ea acid (glutamate) le base (aspartate) ho hydrolysis ka Ca (2+) - mokgoa o itshetlehileng.Mehaho ea mahlakore a mararo ea li-GH92 tse rarahaneng tse nang le li-inhibitors li fana ka temohisiso mabapi le ho khetheha, mokhoa le ho lumellana le leeto la catalysis.Ca(2+) e bapala karolo ea bohlokoa ho thusa ho sotha mannoside hole le boemo ba eona ba fatše (4)C(1) setulong se lebisang sebakeng sa phetoho.（Bibliography: Nat.Chem.Biol.6, 125-32, (2010)
Frontal affinity chromatography ea ovalbumin glycoasparagine kholumong ea concanavalin A-sepharose.Boithuto bo bongata ba boleng bo tlamang ba lectin.
Litšebelisano tsa Sepharose 4B-immobilized concanavalin A (ConA) le li-glycoasparagine tse 10 tse nkiloeng ho ovalbumin li ile tsa fuputsoa ka bongata ke frontal affinity chromatography.Ka mokhoa ona, tharollo ea lik'habohaedreite e sebelisoa ka mokhoa o sa khaotseng ho kholumo ea ConA-Sepharose 'me ho fokotseha ha leseli la pele ho lekanngoa e le parameter ea matla a ho sebelisana.Nako ea ho arohana (Kd) bakeng sa saccharide ka 'ngoe e nang le ConA e ka khethoa.Tlhahlobo ea tlamahano ea p-nitrophenyl-alpha,D-mannoside e bonts'itse hore thepa e tlamang ea ConA ha e fetohe hantle ka mor'a ho se sebetse ho Sepharose 4B.E 'ngoe le e' ngoe ea ovalbumin glycoasparagine e ne e ngotsoe ka tritium ka mokhoa oa reductive methylation bakeng sa tlhahlobo.Papiso ea boleng ba Kd e fumanoeng e bonts'itse hore tlamo ea ConA e fapana haholo ka liphapang tse nyane haholo tsa sebopeho sa ketane ea glycosyl.Liphello li fana ka maikutlo a hore ConA e lemoha sebopeho se khethehileng sa ketane ea glycosyl, Man alpha 1-6(Man alpha 1-3)Man, moo bonyane sehlopha se le seng sa hydroxyl sebakeng sa C-3 sa mannose e amanang le C-6 se lokelang ho lokoloha.
