Lihlahisoa

1.Re ithutile litlamorao tsa inhibitory tsa isorhamnetin ho mushroom tyrosinase ka inhibition kinetics le computational simulation.Isorhamnetin e ile ea thibela tyrosinase ka mokhoa o tsoileng matsoho ka mokhoa o tsoakiloeng ho Ki=0.235±0.013 mM.Litekanyo tsa intrinsic le 1-anilinonaphthalene-8-sulfonate(ANS) -binding fluorescence li bontšitse hore isorhamnetin ha ea ka ea baka liphetoho tse kholo mohahong o phahameng oa tyrosinase.Ho fumana temohisiso mabapi le ts'ebetso ea ho se sebetse, kinetics e ne e baloa ka litekanyo tsa nako ea nako le karabelo e tsoelang pele ea substrate.Liphello li bontšitse hore ho se sebetse ho bakoang ke isorhamnetin e ne e le karabelo ea pele ka mekhoa ea biphasic.Ho fumana temohisiso e eketsehileng, re ile ra etsisa boema-kepe lipakeng tsa tyrosinase le isorhamnetin.Ketsiso e atlehile (matla a tlamang bakeng sa Dock6.3: -32.58 kcal / mol, bakeng sa AutoDock4.2: -5.66 kcal / mol, le bakeng sa Fred2.2: -48.86 kcal / mol), ho fana ka maikutlo a hore isorhamnetin e sebelisana le masala a 'maloa, joalo ka joalo ka HIS244 le MET280.Leano lena la ho sebelisana ha predic ting tyrosinase hammoho le kinetics e thehiloeng ho motsoako oa flavanone e ka 'na ea e-ba molemo ho hlahloba li-inhibitors tsa tlhaho tsa tyrosinase.

2. Acid unfolding pathway of conalbumin (CA), monomeric glycoprotein e tsoang ho lehe la khoho e tšoeu, e batlisisitsoe ka tšebeliso ea CD spectroscopy e hōle le haufi le UV, intrinsic fluorescence emission, extrinsic fluorescence probe 1-anilino-8-napthalene sulfonate (Anilino-8-napthalene sulfonate) le dynamic light scattering (DLS).Re bona liphetoho tse itšetlehileng ka pH sebopehong sa bobeli le sa thuto e phahameng ea CA.E na le sebopeho sa bobeli sa α-helical ho pH 4.0 empa sebopeho sa tahlehelo ho pH 3.0.CA e ne e le teng ka ho khetheha e le boemo ba globule pele ho ho qhibiliha le boemo ba globule e qhibilihisitsoeng ka tharollo ho pH 4.0 le pH 3.0, ka ho latellana.Phello ea pH ho conformation le thermostability ea CA e supa ho hanyetsa mocheso ho pH e sa nke lehlakore.Liphetho tsa DLS li bonts'a hore boemo ba MG bo ne bo le teng e le mofuta o kopaneng oa litharollo tse nang le metsi a nang le hydrodynamic radii ea 4.7 nm.Ho tima tryptophan fluorescence ka acrylamide ho boetse ho netefalitse ho bokellana ha naha e bohareng, e sa senolehang, lipakeng tsa linaha tsa matsoalloa le tse sa phethahalang.