Lihlahisoa

Cysteine ​​(Cys) e bapala karolo ea bohlokoa lits'ebetsong tse ngata tsa 'mele tsa lisele tsa eukaryotic mme ho fumanoa ha eona liseleng ho bohlokoa haholo.Leha ho le joalo, glutathione (GSH), homocysteine ​​​​, N-acetyl-L-cysteine ​​​​le li-thiol tse ling li sitisa haholo ho fumanoa ha Cys.Haholo-holo, GSH e kena-kenana ka matla le ho fumanoa ha lisele tsa Cys (30-200 μM) ka lebaka la khatello ea eona e phahameng ea intracellular (1-10 mM).Mosebetsing ona, ho hlahisoa mochine oa "fluorescent probe" (HOTA) bakeng sa ho lemoha Cys.Probe ena e na le kutlo e ntle haholo le khetho e khotsofatsang bakeng sa ho lemoha Cys ea cellular: ka kenyelletso ea 200 μM Cys, matla a fluorescence a probe (10 μM) a matlafalitsoe ka makhetlo a 117 mme moeli oa ho lemoha o ne o baloa e le 13.47 μM, e leng tlase. ho feta khatello ea cellular Cys;probe e ile ea boela ea lemoha ka ho khetheha 30-200 μM cysteine ​​​​ho feta 1-10 mM glutathione.Ka lebaka leo, liteko tsa litšoantšo tsa lisele li ile tsa etsoa ka probe HOTA.Ho feta moo, liphetho tsa liteko tse thibelang thiol-blocki ng le GSH synthesis inhibiting li netefalitse hore tlhahiso ea intracellular haholo-holo e tsoa ho sebelisana pakeng tsa Cys le HOTA.