Lihlahisoa

Reagent e qhibilihang ka metsi bakeng sa qeto ea enzymatic photometric ea hydrogen peroxide.Li-reagents tse ncha tsa Trinder ke lintho tse tsoang ho aniline tse qhibilihang ka metsing tse sebelisoang haholo litekong tsa tlhahlobo ea mafu le litekong tsa biochemical.Ho na le melemo e 'maloa ho feta li-reagents tse tloaelehileng tsa chromogenic qetong ea colorimetric ea ts'ebetso ea hydrogen peroxide.Li-reagents tse ncha tsa Trinder li tsitsitse ka ho lekana hore li ka sebelisoa ho litharollo le lits'ebetsong tsa liteko tsa lipeipi.Ka pel'a hydrogen peroxide le peroxidase, "reagente ea "Trinder" ea buka e bonts'itsoeng e sebetsana le 4-aminoantipyrine (4-AA) kapa 3-methylbenzothiazole sulfonehydrazone (MBTH) nakong ea karabelo ea oxidative coupling.E etsa li-violets tse tsitsitseng haholo kapa lidae tse putsoa.Motsoako oa molar oa dae o kopantsoeng le MBTH o ne o phahame ka makhetlo a 1.5-2 ho feta oa dae o kopantsoeng le 4-AA;leha ho le joalo, tharollo ea 4-AA e ne e tsitsitse ho feta tharollo ea MBTH.Substrate e na le enzymatic oxidized ka oxidase ea eona ho hlahisa hydrogen peroxide.Motsoako oa hydrogen peroxide o lumellana le motsoako oa substrate.Ka hona, palo ea substrate e ka khethoa ke nts'etsopele ea 'mala oa karabelo ea oxidative coupling.Glucose, joala, acyl-CoA le k'holeseterole li ka sebelisoa ho bona likaroloana tseo li kopantsoeng le buka ea Trinder's reagent le 4-AA.Ho na le li-reagents tse ncha tse 10 tse fumanehang.Har'a li-reagents tse ncha tsa Trinder, TOOS ke eona e sebelisoang haholo.Leha ho le joalo, bakeng sa substrate e itseng, ho hlokahala liteko tse fapaneng tsa li-reagents tsa buka ea Trinder ho nts'etsapele sistimi e nepahetseng ea ho lemoha.

Reagent e qhibilihang ka metsi bakeng sa qeto ea enzymatic photometric ea hydrogen peroxide.Li-reagents tse ncha tsa Trinder ke lintho tse tsoang ho aniline tse qhibilihang ka metsing tse sebelisoang haholo litekong tsa tlhahlobo ea mafu le litekong tsa biochemical.Ho na le melemo e 'maloa ho feta li-reagents tse tloaelehileng tsa chromogenic qetong ea colorimetric ea ts'ebetso ea hydrogen peroxide.Li-reagents tse ncha tsa Trinder li tsitsitse ka ho lekana hore li ka sebelisoa ho litharollo le lits'ebetsong tsa liteko tsa lipeipi.Ka pel'a hydrogen peroxide le peroxidase, "reagente ea "Trinder" ea buka e bonts'itsoeng e sebetsana le 4-aminoantipyrine (4-AA) kapa 3-methylbenzothiazole sulfonehydrazone (MBTH) nakong ea karabelo ea oxidative coupling.E etsa li-violets tse tsitsitseng haholo kapa lidae tse putsoa.Motsoako oa molar oa dae o kopantsoeng le MBTH o ne o phahame ka makhetlo a 1.5-2 ho feta oa dae o kopantsoeng le 4-AA;leha ho le joalo, tharollo ea 4-AA e ne e tsitsitse ho feta tharollo ea MBTH.Substrate e na le enzymatic oxidized ka oxidase ea eona ho hlahisa hydrogen peroxide.Motsoako oa hydrogen peroxide o lumellana le motsoako oa substrate.Ka hona, palo ea substrate e ka khethoa ke nts'etsopele ea 'mala oa karabelo ea oxidative coupling.Glucose, joala, acyl-CoA le k'holeseterole li ka sebelisoa ho bona likaroloana tseo li kopantsoeng le buka ea Trinder's reagent le 4-AA.Ho na le li-reagents tse ncha tse 10 tse fumanehang.Har'a li-reagents tse ncha tsa Trinder, TOOS ke eona e sebelisoang haholo.Leha ho le joalo, bakeng sa substrate e itseng, ho hlokahala liteko tse fapaneng tsa li-reagents tsa buka ea Trinder ho nts'etsapele sistimi e nepahetseng ea ho lemoha.