Lihlahisoa

LecA (PA-IL) ke lectin ea cytotoxic le adhesin e hlahisoang ke Pseudomonas aeruginosa e kopanyang galactosides ea hydrophobic ka ho khetheha le kamano e phahameng.Ka ho sebelisa fusion ea lecA-egfp le tlhahlobo ea immunoblot ea biofilm extracellular matrix, re bonts'a hore lecA e hlahisoa liseleng tse holileng ka biofilm.Litlhahlobong tsa static biofilm ka bobeli polystyrene le tšepe e sa hloekang, botebo ba biofilm le ho koahela holimo ho fokotsoe ke phetoho ea lecA le ho matlafatsoa ke LecA-producing strain PAO-P47.Ho koaheloa ka holim'a biofilm ke khatello ea motsoali, PAO-P47 empa eseng lecA mutant ho li-coupons tsa tšepe e ne e boetse e thibetsoe ke ho hōla ka pel'a isopropyl-beta-D-thiogalactoside (IPTG) kapa p-nitrophenyl-alpha-D-galactoside ( NPG).Ho feta moo, li-biofilm tse holileng tse hlaha tse entsoeng ka bosieong ba li-galactoside tsena tsa hydrophobic li ka qhalakanngoa ka ho eketsoa ha IPTG.Ka lehlakoreng le leng, tlatsetso ea p-nitrophenyl-alpha-L-fucose (NPF) e nang le kamano e phahameng bakeng sa P. aeruginosa LecB (PA-IIL) lecti n e ne e se na phello ho sebopeho sa biofilm kapa ho hasana.Khōlo ea Planktonic ea P. aeruginosa PAO1 ha ea ka ea angoa ke boteng ba IPTG, NPG kapa NPF, leha e le hore mofuta ona o ne o sa khone ho sebelisa tsoekere ena e le mehloli ea khabone, ho fana ka maikutlo a hore liphello tse hlokometsoeng ho thehoa ha biofilm li bakoa ke ho thibela tlhōlisano ea LecA-ligand. tlamo.Liphetho tse ts'oanang li ile tsa boela tsa fumanoa bakeng sa li-biofilms tse hōlileng tlas'a maemo a matla a phallo ho li-coupons tsa tšepe, tse fanang ka maikutlo a hore LecA e kenya letsoho mohahong oa P. aeruginosa biofilm tlas'a maemo a fapaneng a tikoloho.

Alpha-L-fucosidase (EC 3.2.1.51) e khonang ho lokolla masala a t-fucosyl ho tloha ketane e ka thōko ea xyloglucan oligosaccharides e fumanoe makhasi a limela tsa Arabidopsis.Ho feta moo, alpha-L-fucosidase e nang le sebopeho se tšoanang sa substrate e ile ea hloekisoa ho tloha makhasing a k'habeche (Brassica oleracea) ho fana ka sehlopha se le seng ho SDS-PAGE.Ho ile ha fumanoa tatelano tse peli tsa peptide ho tsoa sehlopheng sena sa protheine, 'me li ne li sebelisetsoa ho khetholla mofuta oa liphatsa tsa lefutso tsa Arabidopsis bakeng sa alpha-fucosidase eo re batlang ho e bitsa AtFXG1.Ntle le moo, mofuta oa Arabidopsis o nang le homology e nang le alpha-L-fucosidase e tsebahalang le eona e se e fumanoe, mme re rerile ho e reha lebitso la AtFUC1.Ka bobeli AtFXG1 le ATFUC1 li ile tsa hlahisoa ka mokhoa o fapaneng ka har'a lisele tsa Pichia pastoris le mesebetsi ea alpha-L-fucosidase e patiloeng molemong oa setso.Alpha-L-fucosidase e kentsoeng ke AtFXG1 e ne e sebetsa khahlanong le oligosaccharides ho tloha xyloglucan XXFG hammoho le khahlanong le 2'-fucosyl-lactitol empa eseng khahlanong le p-nitrophenyl-alpha-L-fucopyranoside.Leha ho le joalo, AtFUC1 e hlahisitsoeng ka mokhoa o fapaneng e ne e sebetsa khahlanong le 2'-fucosyl-lactitol feela.Kahoo, ea pele e tlameha ho amana le metabolism ea xyloglucan.