NSP-AS CAS: 211106-69-3 Yellow crystaline phofo
Nomoro ea lethathamo | XD90128 |
Lebitso la Sehlahiswa | 3-[9-((3-(carboxypropyl))[4-methxylphenyl]\sulfonyl)amine)carboxyl] -10-acridiniumyl) -1-propanesulfonate letsoai le ka hare |
CAS | 211106-69-3 |
Foromo ea limolek'hule | C28H28N2O8S2 |
Boima ba Molek'hule | 584.661 |
Lintlha tsa polokelo | 2 ho isa ho 8 °C |
Tlhaloso ea Sehlahisoa
Ponahalo | Yellow crystaline phofo |
Tlhahlobo | 99% |
Physicochemical properties The dilute tharollo ea acridine le matsoai a eona a bontša pherese kapa tala fluorescence.Litharollo tse hlapollang tsa letsoai li na le fluorescence e tala, 'me ha li hlapolotsoe hape, ka lebaka la hydrolysis ea letsoai, li fetoha li-acridines tse sa lefelloeng, tse bontšang fluorescence e pherese.Mokelikeli o nang le metsi o na le alkaline e fokolang 'me e kopana le li-inorganic acid ho etsa letsoai.Acridine e tsitsitse haholo, sebopeho sa eona se tšoana le anthracene, 'me lik'hemik'hale tsa eona li boetse li tšoana haholo.Ka bobeli mouoane le tharollo lia halefisa, li halefisa ka matla letlalo le lera la mucous, 'me ho hema mouoane ho ka baka ho khohlela.
E le probe e khanyang, e sebelisoa thutong ea liphatsa tsa lefutso.Karabelo e ngotsoe ka acridan (9,10-dihydroacridine) e le substrate le alkaline phosphatase, e hlahisang chemiluminescence e tsitsitseng e phahameng.E fana ka kutloisiso e phahameng le boiketlo ba tšebeliso ea alkaline phosphatase conjugation nakong ea ho lemoha chemiluminescent.Alkaline phosphatase e fumanoa ka tlase ho 10-19 mol, e phahama ka potlako ho fokotsa nako ea ho lemoha le ho eketsa ho feta, 'me moepa oa curve ea calibration ea linear o reriloe ka logarithmic e lekanang le 1.0.Palo e le 'ngoe kapa ho feta ea enzyme e hlahisa palo e le' ngoe kapa ho feta ea luminescence, luminescence e tsoelang pele-e sa hlokeng nako ea teko.Luminescence intensity e ka baloa ho tloha mokokotlong o hlahisitsoeng oa linear calibration ka nako leha e le efe, 'me liphetho tsa tlhahlobo ha li amehe mocheso oa 22 ° C - 35 ° C, ho fokotsa ho nepahala ho hlokahalang ho laola mocheso.
Kopo: E ka sebelisoa bakeng sa ho ngola liprotheine, li-antigen, li-antibodies, nucleic acids (DNA, RNA), joalo-joalo.