Lihlahisoa

Ka mokhoa oa kinetic angiotensin-converting enzyme (ACE), buffer e sebetsang le e nepahetseng ke 80 mmol/L borate buffer pH 8.2 (37 degrees C).Karolo ea lag e bonoa karabelong, 'me ho etsoa tlhahiso ea metsotso e 5 ho kenya substrate le plasma pele ho tekanyo ea kinetic.The substrate, N-[3-(2-furyl)acryloyl] -L-phenylalanylglycylglycine (FAPGG), mahloriso a phahamisetsoa ho 1.0 mmol/L le wavelength ea tekanyo e ho 345 nm ho netefatsa hore ho na le moeli oa tekanyo.Mokhoa o reriloeng o sebelisa karo-karolelano ea molumo oa plasma-to-reagent ea 1: 9.Mefuta e fapaneng ea tlhahlobo e fetela ho hoo e ka bang 170 U/L, e emelang 25% substrate hydrolysis.Ho monyela ha FAPGG ho khethoa ka ho lekanya phapang pakeng tsa 1.0 mmol/L FAPGG le tharollo ea sehlahisoa.Botšepehi ba maqhubu a maqhubu bo hlahlojoa ka ho hlokomela boleng bo lebeletsoeng ba ho monya tharollo ea FAPGG, 'me ho kheloha ha 1.0-nm ho tloha 345 nm ho fetola ho monya ka 15.5%.Ho nepahala ha litlhahlobo tsa ACE hoo e ka bang 60 le 100 U/L ke 3.5% le 2.4% ka har'a betch le 2.9% le 2.6% lipakeng tsa batch, ka ho latellana.Nako ea litšupiso (2.5th ho ea ho 97.5th percentile) ke 41-139 U/L, 'me ha ho na phapang pakeng tsa litekanyetso tsa banna le basali.