Lihlahisoa

MES Buffer e Ama Arabidopsis Root Apex Zonation le Kholo ea Motso ka ho hatella Moloko oa Superoxide ho Root Apex

Limeleng, kholo ea metso le moriri oa metso e laoloa ke taolo e ntle ea lisele tsa pH le mefuta ea oksijene e sebetsang (ROS).MES, 2-(N-morpholino) ethanesulfonic acid joalo ka e 'ngoe ea li-buffers tsa Good e se e sebelisoa ka bophara molemong oa ho thibela limela,' me ho nahanoa hore e tšoanela kholo ea semela ka ho tsepamisa mohopolo ho 0.1% (w/v) hobane matla a buffer MES ho tloha pH 5.5-7.0 (bakeng sa Arabidopsis, pH 5.8).Leha ho le joalo, litlaleho tse ngata li bontšitse hore, ka tlhaho, metso e hloka litekanyetso tse fapaneng tsa pH holim'a libaka tse khethehileng tsa metso, e leng meristem, transition zone, le elongation zone.Leha taba ea hore metso e lula e hola mecheng ea litaba e nang le molek'hule ea buffer, ha ho tsejoe hakaalo ka phello ea MES kholong ea metso.Mona, re hlahlobile litlamorao tsa likhakanyo tse fapaneng tsa buffer ea MES re sebelisa metso e ntseng e hola ea Arabidopsis thaliana.Liphetho tsa rona li bonts'a hore 1% ea MES e sitisitse kholo ea metso haholo, palo ea moriri oa metso le bolelele ba meristem, athe 0.1% e khothalelitse kholo ea metso le sebaka se phahameng sa metso (sebaka se tlohang ntlheng ea motso ho ea sebakeng sa phetoho).Ho feta moo, tlhahiso ea superoxide ka har'a metso e ile ea nyamela ho 1% ea MES.Liphetho tsena li fana ka maikutlo a hore MES e sitisa motso o tloaelehileng oa morphogenesis ka ho fetola ROS homeostasis ka holimo ho metso.