L-Proline Cas: 147-85-3 99% phofo e tšoeu
| Nomoro ea lethathamo | XD90293 |
| Lebitso la Sehlahiswa | L-Proline |
| CAS | 147-85-3 |
| Foromo ea limolek'hule | C5H9NO2 |
| Boima ba Molek'hule | 115.13046 |
| Lintlha tsa polokelo | Ambient |
| Khoutu ea Lekhetho e Kopantsoeng | 29339980 |
Tlhaloso ea Sehlahisoa
| Tlhahlobo | 99%mets |
| Ponahalo | Phofo e tšoeu |
| Ho potoloha ho itseng | -84,5 ho ea ho -86 |
| Litšepe tse boima | <15ppm |
| AS | <1ppm |
| Ph | 5.9 - 6,9 |
| SO4 | <0.050% |
| Fe | <30ppm |
| Tahlehelo ka ho Omisa | <0.3% |
| Masalla a ho Ignition | <0.10% |
| NH4 | <0.02% |
| Cl | <0.050% |
| Boemo ba Tharollo | >98% |
Ho utloisisa metabolism ea moamoheli oa likokoana-hloko ho bohlokoa bakeng sa nts'etsopele le ntlafatso ea lits'ebetso tsa biocatalytic ea lisele tsohle, kaha e laela katleho ea tlhahiso.Sena ke 'nete haholo-holo bakeng sa redox biocatalysis moo lisele tse sebetsang hantle tsa metabolism li sebelisoang ka lebaka la cofactor/cosubstrate regenerative capacity ea endogenous ho moamoheli.Recombinant Escherichia coli e ne e sebelisetsoa ho hlahisa proline-4-hydroxylase (P4H), e leng dioxygenase e susumetsang hydroxylation ea L-proline ea mahala ho trans-4-hydroxy-L-proline le a-ketoglutarate (a-KG) e le cosubstrate.Ho biocatalyst ena ea lisele tsohle, metabolism ea carbon e bohareng e fana ka cosubstrate a-KG e hlokahalang, e kopanyang ts'ebetso ea P4H ea biocatalytic ka ho toba ho carbon metabolism le mosebetsi oa metabolic.Ka ho sebelisa lisebelisoa tse peli tsa liteko le tsa computational biology, joalo ka boenjiniere ba metabolism le (13)C-metabolic flux analysis ((13)C-MFA), re ile ra etsa lipatlisiso le ho hlalosa ka bongata karabelo ea fisioloji, metabolic, le bioenergetic ea biocatalyst ea sele eohle. ho bioconversion e lebisitsoeng le ho tsebahatsa litšitiso tse ka bang teng tsa metabolic bakeng sa boenjiniere bo eketsehileng ba tsela e utloahalang.Tlhahiso ea proline e haelloang ke E. coli e ile ea etsoa ka ho hlakola putA gene encoding proline dehydrogenase.Li-biotransformations tsa lisele tse feletseng tse nang le mofuta ona oa phetoho li lebisitse ho proline hydroxylation feela empa hape le ho imena habeli ha sekhahla sa sebopeho sa trans-4-L-hydroxyproline (hyp), ha se bapisoa le mofuta o hlaha.Tlhahlobo ea carbon flux ka metabolism e bohareng ea khatello ea mutant e senoletse hore tlhokahalo e eketsehileng ea a-KG bakeng sa ts'ebetso ea P4H ha ea ka ea ntlafatsa phallo e hlahisang a-KG, e bontšang ts'ebetso ea potoloho ea TCA e laoloang ka thata tlas'a maemo a ithutoang.Ka har'a mefuta e hlaha, synthesis ea P4H le catalysis li bakile phokotso ea lihlahisoa tsa biomass.Ho khahlisang, mofuta oa ΔputA o ile oa lefa hape tahlehelo e amanang le ATP le NADH ka ho fokotsa litlhoko tsa matla a tlhokomelo ka litekanyetso tse tlase tsa ho monya tsoekere, ho fapana le ho eketsa ts'ebetso ea TCA. The putA knockout in recombinant E. coli BL21(DE3)(pLysS) e fumanoe ho ba le ts'episo bakeng sa tlhahiso ea tlhahiso ea P4H catalysis eseng feela ka tlhahiso ea biotransformation, empa hape mabapi le litekanyetso tsa biotransformation le proline uptake le lihlahisoa tsa hyp mohloling oa matla.Liphetho li bonts'a hore, ka mor'a ho kokota ha putA, ho kopanngoa ha TCA-cycle ho proline hydroxylation ka cosubstrate a-KG e fetoha ntho ea bohlokoa e thibelang le sepheo sa ho ntlafatsa ts'ebetso ea li-biotransformations tse itšetlehileng ka-KG.
