HEPBS Cas:161308-36-7 N- (2- Hydroxyethyl) piperazine- N'- (4- butanesulfonic acid) phofo e tšoeu ea crystalline 99%
Nomoro ea lethathamo | XD90100 |
Lebitso la Sehlahiswa | HEPBS |
CAS | 161308-36-7 |
Foromo ea limolek'hule | Setšoantšo sa C10H22N2O4S |
Boima ba Molek'hule | 266.36 |
Lintlha tsa polokelo | Ambient |
Khoutu ea Lekhetho e Kopantsoeng | 2933599090 |
Tlhaloso ea Sehlahisoa
Ponahalo | Phofo e tšoeu ea kristale |
Assay | ≥ 99% |
Mocheso oa polokelo | Lebenkele ho RT |
Sebaka se qhibilihang | 211-216°C |
Acidity coefficient (pKa) | 8.3(ho 25℃) |
Re utloisisa bohlokoa ba ho sireletsa botšepehi ba li-biomolecule tsa hau le li-reagents tse nang le litsamaiso tse nepahetseng tsa ho boloka.Li-buffers tsa rona tsa baeloji li fana ka botsitso ba tharollo le taolo ea pH ntle le ho kena-kenana le lits'ebetso tsa tlhaho, hape li fana ka matsoai a bohlokoa le limatlafatsi bakeng sa lisele le lisele.Sistimi ea rona e tsoetseng pele ea ho thibela likokoana-hloko e ka u tlisetsa botsitso bo makatsang moetlong oa lisele, polymerase chain reaction (PCR), tlhahlobo ea lithethefatsi, bioprocessing, tlhoekiso, le lits'ebetso tsa ho qetela tsa tlhahiso.Lihlahisoa tsohle tsa rona li ka fokotsoa ho tloha lipatlisisong tsa pele ho isa lits'ebetsong tsa khoebo ka limaraka tse fapaneng tsa buffer.Re fana ka litlhophiso tse ngata tsa liphutheloana, ho kopanya tloaelo, esita le mokhoa oa metsi.
Re boetse re fana ka khetho e felletseng ea lik'hemik'hale tsa boleng bo holimo tsa biochemical ka mefuta e fapaneng ea limaraka le likhethong tse fapaneng tsa boiqapelo, tse bonolo tsa ho paka.Ntle le lihlahisoa tse kantle ho sethala, re fana ka tlhahiso ea reagent e tloaelehileng, ho kopanya le ho paka ho fihlela litlhoko tsa hau.
Molao-motheo oa ts'ebetso le boleng ba pH ba tharollo ea "buffer" Ha palo e itseng ea asiti le alkali e eketsoa litharollong tse ling, e na le phello ea ho sitisa phetoho ea pH ea tharollo, e bitsoang buffering effect.Tharollo e joalo e bitsoa tharollo ea buffer.Litharollo tse tsoakiloeng tsa li-acid tse fokolang le matsoai a tsona (joaloka HAc le NaAc), le litharollo tse tsoakiloeng tsa metheo e fokolang le matsoai a tsona (joalo ka NH3 · H2O le NH4Cl) ke tharollo ea buffer.Tšusumetso ea ho thibela tharollo e entsoeng ka acid e fokolang HA le letsoai la eona la NaA holim'a asiti e bakoa ke ho ba teng ha palo e lekaneng ea alkali A- tharollong.Ha palo e itseng ea asiti e matla e eketsoa tharollong ena, H ions ha e le hantle e jeoa ke A-ion: kahoo pH ea tharollo e batla e sa fetohe;ha palo e itseng ea motheo e matla e eketsoa, acid e fokolang HA e teng ka tharollo e ja li-OH-ion e sitisa phetoho ea pH.